Abstract
The human buccal micronucleus cytome assay (BMCyt) is one of the most widely used techniques to measure genetic damage in human population studies. Reducing protocol variability, assessing the role of confounders, and estimating a range of reference values are research priorities that will be addressed by the HUMN XL collaborative study. The HUMN XL project evaluates the impact of host factors, occupation, life-style, disease status, and protocol features on the occurrence of MN in exfoliated buccal cells. In addition, the study will provide a range of reference values for all cytome endpoints. A database of 5424 subjects with buccal MN values obtained from 30 laboratories worldwide was compiled and analyzed to investigate the influence of several conditions affecting MN frequency. Random effects models were mostly used to investigate MN predictors. The estimated spontaneous MN frequency was 0.74‰ (95% CI 0.52-1.05). Only staining among technical features influenced MN frequency, with an abnormal increase for non-DNA-specific stains. No effect of gender was evident, while the trend for age was highly significant (pXL project identified priorities for validation studies, increased the basic knowledge of the assay, and contributed to the creation of a laboratory network which in perspective may allow the evaluation of disease risk associated with MN frequency.
| Original language | English |
|---|---|
| Pages (from-to) | 88-97 |
| Number of pages | 10 |
| Journal | Mutation Research - Reviews in Mutation Research |
| Volume | 728 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Nov 2011 |
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Keywords
- Assay standardization
- Buccal micronucleus cytome assay (BMCyt)
- Exfoliated cell
- Genetic instability
- Molecular epidemiology
- Pooled analysis
ASJC Scopus subject areas
- Genetics
- Health, Toxicology and Mutagenesis
Cite this
The HUman MicroNucleus project on eXfoLiated buccal cells (HUMN XL) : The role of life-style, host factors, occupational exposures, health status, and assay protocol. / Bonassi, Stefano; Coskun, Erdem; Ceppi, Marcello; Lando, Cecilia; Bolognesi, Claudia; Burgaz, Sema; Holland, Nina; Kirsh-Volders, Micheline; Knasmueller, Siegfried; Zeiger, Errol; Carnesoltas, Deyanira; Cavallo, Delia; da Silva, Juliana; de Andrade, Vanessa M.; Demircigil, Gonca Cakmak; Odio, Aníbal Domínguez; Donmez-Altuntas, Hamiyet; Gattas, Gilka; Giri, Ashok; Giri, Sarbani; Gómez-Meda, Belinda; Gómez-Arroyo, Sandra; Hadjidekova, Valeria; Haveric, Anja; Kamboj, Mala; Kurteshi, Kemajl; Martino-Roth, Maria Grazia; Montoya, Regina Montero; Nersesyan, Armen; Pastor-Benito, Susana; Salvadori, Daisy Maria Favero; Shaposhnikova, Alina; Stopper, Helga; Thomas, Philip; Torres-Bugarín, Olivia; Yadav, Abhay Singh; González, Guillermo Zúñiga; Fenech, Michael.
In: Mutation Research - Reviews in Mutation Research, Vol. 728, No. 3, 11.2011, p. 88-97.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The HUman MicroNucleus project on eXfoLiated buccal cells (HUMN XL)
T2 - The role of life-style, host factors, occupational exposures, health status, and assay protocol
AU - Bonassi, Stefano
AU - Coskun, Erdem
AU - Ceppi, Marcello
AU - Lando, Cecilia
AU - Bolognesi, Claudia
AU - Burgaz, Sema
AU - Holland, Nina
AU - Kirsh-Volders, Micheline
AU - Knasmueller, Siegfried
AU - Zeiger, Errol
AU - Carnesoltas, Deyanira
AU - Cavallo, Delia
AU - da Silva, Juliana
AU - de Andrade, Vanessa M.
AU - Demircigil, Gonca Cakmak
AU - Odio, Aníbal Domínguez
AU - Donmez-Altuntas, Hamiyet
AU - Gattas, Gilka
AU - Giri, Ashok
AU - Giri, Sarbani
AU - Gómez-Meda, Belinda
AU - Gómez-Arroyo, Sandra
AU - Hadjidekova, Valeria
AU - Haveric, Anja
AU - Kamboj, Mala
AU - Kurteshi, Kemajl
AU - Martino-Roth, Maria Grazia
AU - Montoya, Regina Montero
AU - Nersesyan, Armen
AU - Pastor-Benito, Susana
AU - Salvadori, Daisy Maria Favero
AU - Shaposhnikova, Alina
AU - Stopper, Helga
AU - Thomas, Philip
AU - Torres-Bugarín, Olivia
AU - Yadav, Abhay Singh
AU - González, Guillermo Zúñiga
AU - Fenech, Michael
PY - 2011/11
Y1 - 2011/11
N2 - The human buccal micronucleus cytome assay (BMCyt) is one of the most widely used techniques to measure genetic damage in human population studies. Reducing protocol variability, assessing the role of confounders, and estimating a range of reference values are research priorities that will be addressed by the HUMN XL collaborative study. The HUMN XL project evaluates the impact of host factors, occupation, life-style, disease status, and protocol features on the occurrence of MN in exfoliated buccal cells. In addition, the study will provide a range of reference values for all cytome endpoints. A database of 5424 subjects with buccal MN values obtained from 30 laboratories worldwide was compiled and analyzed to investigate the influence of several conditions affecting MN frequency. Random effects models were mostly used to investigate MN predictors. The estimated spontaneous MN frequency was 0.74‰ (95% CI 0.52-1.05). Only staining among technical features influenced MN frequency, with an abnormal increase for non-DNA-specific stains. No effect of gender was evident, while the trend for age was highly significant (pXL project identified priorities for validation studies, increased the basic knowledge of the assay, and contributed to the creation of a laboratory network which in perspective may allow the evaluation of disease risk associated with MN frequency.
AB - The human buccal micronucleus cytome assay (BMCyt) is one of the most widely used techniques to measure genetic damage in human population studies. Reducing protocol variability, assessing the role of confounders, and estimating a range of reference values are research priorities that will be addressed by the HUMN XL collaborative study. The HUMN XL project evaluates the impact of host factors, occupation, life-style, disease status, and protocol features on the occurrence of MN in exfoliated buccal cells. In addition, the study will provide a range of reference values for all cytome endpoints. A database of 5424 subjects with buccal MN values obtained from 30 laboratories worldwide was compiled and analyzed to investigate the influence of several conditions affecting MN frequency. Random effects models were mostly used to investigate MN predictors. The estimated spontaneous MN frequency was 0.74‰ (95% CI 0.52-1.05). Only staining among technical features influenced MN frequency, with an abnormal increase for non-DNA-specific stains. No effect of gender was evident, while the trend for age was highly significant (pXL project identified priorities for validation studies, increased the basic knowledge of the assay, and contributed to the creation of a laboratory network which in perspective may allow the evaluation of disease risk associated with MN frequency.
KW - Assay standardization
KW - Buccal micronucleus cytome assay (BMCyt)
KW - Exfoliated cell
KW - Genetic instability
KW - Molecular epidemiology
KW - Pooled analysis
UR - http://www.scopus.com/inward/record.url?scp=80053592718&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80053592718&partnerID=8YFLogxK
U2 - 10.1016/j.mrrev.2011.06.005
DO - 10.1016/j.mrrev.2011.06.005
M3 - Article
C2 - 21763453
AN - SCOPUS:80053592718
VL - 728
SP - 88
EP - 97
JO - Mutation Research - Reviews in Mutation Research
JF - Mutation Research - Reviews in Mutation Research
SN - 1383-5742
IS - 3
ER -