The HUman MicroNucleus project on eXfoLiated buccal cells (HUMN XL): The role of life-style, host factors, occupational exposures, health status, and assay protocol

Stefano Bonassi; Erdem Coskun; Marcello Ceppi; Cecilia Lando; Claudia Bolognesi; Sema Burgaz; Nina Holland; Micheline Kirsh-Volders; Siegfried Knasmueller; Errol Zeiger; Deyanira Carnesoltas; Delia Cavallo; Juliana da Silva; Vanessa M. de Andrade; Gonca Cakmak Demircigil; Aníbal Domínguez Odio; Hamiyet Donmez-Altuntas; Gilka Gattas; Ashok Giri; Sarbani Giri; Belinda Gómez-Meda; Sandra Gómez-Arroyo; Valeria Hadjidekova; Anja Haveric; Mala Kamboj; Kemajl Kurteshi; Maria Grazia Martino-Roth; Regina Montero Montoya; Armen Nersesyan; Susana Pastor-Benito; Daisy Maria Favero Salvadori; Alina Shaposhnikova; Helga Stopper; Philip Thomas; Olivia Torres-Bugarín; Abhay Singh Yadav; Guillermo Zúñiga González; Michael Fenech

doi:10.1016/j.mrrev.2011.06.005

The HUman MicroNucleus project on eXfoLiated buccal cells (HUMN _XL): The role of life-style, host factors, occupational exposures, health status, and assay protocol

Stefano Bonassi, Erdem Coskun, Marcello Ceppi, Cecilia Lando, Claudia Bolognesi, Sema Burgaz, Nina Holland, Micheline Kirsh-Volders, Siegfried Knasmueller, Errol Zeiger, Deyanira Carnesoltas, Delia Cavallo, Juliana da Silva, Vanessa M. de Andrade, Gonca Cakmak Demircigil, Aníbal Domínguez Odio, Hamiyet Donmez-Altuntas, Gilka Gattas, Ashok Giri, Sarbani GiriBelinda Gómez-Meda, Sandra Gómez-Arroyo, Valeria Hadjidekova, Anja Haveric, Mala Kamboj, Kemajl Kurteshi, Maria Grazia Martino-Roth, Regina Montero Montoya, Armen Nersesyan, Susana Pastor-Benito, Daisy Maria Favero Salvadori, Alina Shaposhnikova, Helga Stopper, Philip Thomas, Olivia Torres-Bugarín, Abhay Singh Yadav, Guillermo Zúñiga González, Michael Fenech

Research output: Contribution to journal › Article › peer-review

Abstract

The human buccal micronucleus cytome assay (BMCyt) is one of the most widely used techniques to measure genetic damage in human population studies. Reducing protocol variability, assessing the role of confounders, and estimating a range of reference values are research priorities that will be addressed by the HUMN _XL collaborative study. The HUMN _XL project evaluates the impact of host factors, occupation, life-style, disease status, and protocol features on the occurrence of MN in exfoliated buccal cells. In addition, the study will provide a range of reference values for all cytome endpoints. A database of 5424 subjects with buccal MN values obtained from 30 laboratories worldwide was compiled and analyzed to investigate the influence of several conditions affecting MN frequency. Random effects models were mostly used to investigate MN predictors. The estimated spontaneous MN frequency was 0.74‰ (95% CI 0.52-1.05). Only staining among technical features influenced MN frequency, with an abnormal increase for non-DNA-specific stains. No effect of gender was evident, while the trend for age was highly significant (pXL project identified priorities for validation studies, increased the basic knowledge of the assay, and contributed to the creation of a laboratory network which in perspective may allow the evaluation of disease risk associated with MN frequency.

Original language	English
Pages (from-to)	88-97
Number of pages	10
Journal	Mutation Research - Reviews in Mutation Research
Volume	728
Issue number	3
DOIs	https://doi.org/10.1016/j.mrrev.2011.06.005
Publication status	Published - Nov 2011

Keywords

Assay standardization
Buccal micronucleus cytome assay (BMCyt)
Exfoliated cell
Genetic instability
Molecular epidemiology
Pooled analysis

ASJC Scopus subject areas

Genetics
Health, Toxicology and Mutagenesis

Access to Document

10.1016/j.mrrev.2011.06.005

Cite this

Bonassi, S., Coskun, E., Ceppi, M., Lando, C., Bolognesi, C., Burgaz, S., Holland, N., Kirsh-Volders, M., Knasmueller, S., Zeiger, E., Carnesoltas, D., Cavallo, D., da Silva, J., de Andrade, V. M., Demircigil, G. C., Odio, A. D., Donmez-Altuntas, H., Gattas, G., Giri, A., ... Fenech, M. (2011). The HUman MicroNucleus project on eXfoLiated buccal cells (HUMN _XL): The role of life-style, host factors, occupational exposures, health status, and assay protocol. Mutation Research - Reviews in Mutation Research, 728(3), 88-97. https://doi.org/10.1016/j.mrrev.2011.06.005

The HUman MicroNucleus project on eXfoLiated buccal cells (HUMN _XL) : The role of life-style, host factors, occupational exposures, health status, and assay protocol. / Bonassi, Stefano; Coskun, Erdem; Ceppi, Marcello et al.

In: Mutation Research - Reviews in Mutation Research, Vol. 728, No. 3, 11.2011, p. 88-97.

Research output: Contribution to journal › Article › peer-review

Bonassi, S, Coskun, E, Ceppi, M, Lando, C, Bolognesi, C, Burgaz, S, Holland, N, Kirsh-Volders, M, Knasmueller, S, Zeiger, E, Carnesoltas, D, Cavallo, D, da Silva, J, de Andrade, VM, Demircigil, GC, Odio, AD, Donmez-Altuntas, H, Gattas, G, Giri, A, Giri, S, Gómez-Meda, B, Gómez-Arroyo, S, Hadjidekova, V, Haveric, A, Kamboj, M, Kurteshi, K, Martino-Roth, MG, Montoya, RM, Nersesyan, A, Pastor-Benito, S, Salvadori, DMF, Shaposhnikova, A, Stopper, H, Thomas, P, Torres-Bugarín, O, Yadav, AS, González, GZ & Fenech, M 2011, 'The HUman MicroNucleus project on eXfoLiated buccal cells (HUMN _XL): The role of life-style, host factors, occupational exposures, health status, and assay protocol', Mutation Research - Reviews in Mutation Research, vol. 728, no. 3, pp. 88-97. https://doi.org/10.1016/j.mrrev.2011.06.005

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abstract = "The human buccal micronucleus cytome assay (BMCyt) is one of the most widely used techniques to measure genetic damage in human population studies. Reducing protocol variability, assessing the role of confounders, and estimating a range of reference values are research priorities that will be addressed by the HUMN XL collaborative study. The HUMN XL project evaluates the impact of host factors, occupation, life-style, disease status, and protocol features on the occurrence of MN in exfoliated buccal cells. In addition, the study will provide a range of reference values for all cytome endpoints. A database of 5424 subjects with buccal MN values obtained from 30 laboratories worldwide was compiled and analyzed to investigate the influence of several conditions affecting MN frequency. Random effects models were mostly used to investigate MN predictors. The estimated spontaneous MN frequency was 0.74‰ (95% CI 0.52-1.05). Only staining among technical features influenced MN frequency, with an abnormal increase for non-DNA-specific stains. No effect of gender was evident, while the trend for age was highly significant (pXL project identified priorities for validation studies, increased the basic knowledge of the assay, and contributed to the creation of a laboratory network which in perspective may allow the evaluation of disease risk associated with MN frequency.",

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AU - Coskun, Erdem

AU - Ceppi, Marcello

AU - Lando, Cecilia

AU - Bolognesi, Claudia

AU - Burgaz, Sema

AU - Holland, Nina

AU - Kirsh-Volders, Micheline

AU - Knasmueller, Siegfried

AU - Zeiger, Errol

AU - Carnesoltas, Deyanira

AU - Cavallo, Delia

AU - da Silva, Juliana

AU - de Andrade, Vanessa M.

AU - Demircigil, Gonca Cakmak

AU - Odio, Aníbal Domínguez

AU - Donmez-Altuntas, Hamiyet

AU - Gattas, Gilka

AU - Giri, Ashok

AU - Giri, Sarbani

AU - Gómez-Meda, Belinda

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AU - Hadjidekova, Valeria

AU - Haveric, Anja

AU - Kamboj, Mala

AU - Kurteshi, Kemajl

AU - Martino-Roth, Maria Grazia

AU - Montoya, Regina Montero

AU - Nersesyan, Armen

AU - Pastor-Benito, Susana

AU - Salvadori, Daisy Maria Favero

AU - Shaposhnikova, Alina

AU - Stopper, Helga

AU - Thomas, Philip

AU - Torres-Bugarín, Olivia

AU - Yadav, Abhay Singh

AU - González, Guillermo Zúñiga

AU - Fenech, Michael

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