The HUman MicroNucleus project on eXfoLiated buccal cells (HUMN XL): The role of life-style, host factors, occupational exposures, health status, and assay protocol

Stefano Bonassi, Erdem Coskun, Marcello Ceppi, Cecilia Lando, Claudia Bolognesi, Sema Burgaz, Nina Holland, Micheline Kirsh-Volders, Siegfried Knasmueller, Errol Zeiger, Deyanira Carnesoltas, Delia Cavallo, Juliana da Silva, Vanessa M. de Andrade, Gonca Cakmak Demircigil, Aníbal Domínguez Odio, Hamiyet Donmez-Altuntas, Gilka Gattas, Ashok Giri, Sarbani GiriBelinda Gómez-Meda, Sandra Gómez-Arroyo, Valeria Hadjidekova, Anja Haveric, Mala Kamboj, Kemajl Kurteshi, Maria Grazia Martino-Roth, Regina Montero Montoya, Armen Nersesyan, Susana Pastor-Benito, Daisy Maria Favero Salvadori, Alina Shaposhnikova, Helga Stopper, Philip Thomas, Olivia Torres-Bugarín, Abhay Singh Yadav, Guillermo Zúñiga González, Michael Fenech

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

The human buccal micronucleus cytome assay (BMCyt) is one of the most widely used techniques to measure genetic damage in human population studies. Reducing protocol variability, assessing the role of confounders, and estimating a range of reference values are research priorities that will be addressed by the HUMN XL collaborative study. The HUMN XL project evaluates the impact of host factors, occupation, life-style, disease status, and protocol features on the occurrence of MN in exfoliated buccal cells. In addition, the study will provide a range of reference values for all cytome endpoints. A database of 5424 subjects with buccal MN values obtained from 30 laboratories worldwide was compiled and analyzed to investigate the influence of several conditions affecting MN frequency. Random effects models were mostly used to investigate MN predictors. The estimated spontaneous MN frequency was 0.74‰ (95% CI 0.52-1.05). Only staining among technical features influenced MN frequency, with an abnormal increase for non-DNA-specific stains. No effect of gender was evident, while the trend for age was highly significant (pXL project identified priorities for validation studies, increased the basic knowledge of the assay, and contributed to the creation of a laboratory network which in perspective may allow the evaluation of disease risk associated with MN frequency.

Original languageEnglish
Pages (from-to)88-97
Number of pages10
JournalMutation Research - Reviews in Mutation Research
Volume728
Issue number3
DOIs
Publication statusPublished - Nov 2011

Fingerprint

Cheek
Occupational Health
Occupational Exposure
Health Status
Life Style
Reference Values
Micronucleus Tests
Validation Studies
Occupations
Coloring Agents
Databases
Staining and Labeling
Research
Population

Keywords

  • Assay standardization
  • Buccal micronucleus cytome assay (BMCyt)
  • Exfoliated cell
  • Genetic instability
  • Molecular epidemiology
  • Pooled analysis

ASJC Scopus subject areas

  • Genetics
  • Health, Toxicology and Mutagenesis

Cite this

The HUman MicroNucleus project on eXfoLiated buccal cells (HUMN XL) : The role of life-style, host factors, occupational exposures, health status, and assay protocol. / Bonassi, Stefano; Coskun, Erdem; Ceppi, Marcello; Lando, Cecilia; Bolognesi, Claudia; Burgaz, Sema; Holland, Nina; Kirsh-Volders, Micheline; Knasmueller, Siegfried; Zeiger, Errol; Carnesoltas, Deyanira; Cavallo, Delia; da Silva, Juliana; de Andrade, Vanessa M.; Demircigil, Gonca Cakmak; Odio, Aníbal Domínguez; Donmez-Altuntas, Hamiyet; Gattas, Gilka; Giri, Ashok; Giri, Sarbani; Gómez-Meda, Belinda; Gómez-Arroyo, Sandra; Hadjidekova, Valeria; Haveric, Anja; Kamboj, Mala; Kurteshi, Kemajl; Martino-Roth, Maria Grazia; Montoya, Regina Montero; Nersesyan, Armen; Pastor-Benito, Susana; Salvadori, Daisy Maria Favero; Shaposhnikova, Alina; Stopper, Helga; Thomas, Philip; Torres-Bugarín, Olivia; Yadav, Abhay Singh; González, Guillermo Zúñiga; Fenech, Michael.

In: Mutation Research - Reviews in Mutation Research, Vol. 728, No. 3, 11.2011, p. 88-97.

Research output: Contribution to journalArticle

Bonassi, S, Coskun, E, Ceppi, M, Lando, C, Bolognesi, C, Burgaz, S, Holland, N, Kirsh-Volders, M, Knasmueller, S, Zeiger, E, Carnesoltas, D, Cavallo, D, da Silva, J, de Andrade, VM, Demircigil, GC, Odio, AD, Donmez-Altuntas, H, Gattas, G, Giri, A, Giri, S, Gómez-Meda, B, Gómez-Arroyo, S, Hadjidekova, V, Haveric, A, Kamboj, M, Kurteshi, K, Martino-Roth, MG, Montoya, RM, Nersesyan, A, Pastor-Benito, S, Salvadori, DMF, Shaposhnikova, A, Stopper, H, Thomas, P, Torres-Bugarín, O, Yadav, AS, González, GZ & Fenech, M 2011, 'The HUman MicroNucleus project on eXfoLiated buccal cells (HUMN XL): The role of life-style, host factors, occupational exposures, health status, and assay protocol', Mutation Research - Reviews in Mutation Research, vol. 728, no. 3, pp. 88-97. https://doi.org/10.1016/j.mrrev.2011.06.005
Bonassi, Stefano ; Coskun, Erdem ; Ceppi, Marcello ; Lando, Cecilia ; Bolognesi, Claudia ; Burgaz, Sema ; Holland, Nina ; Kirsh-Volders, Micheline ; Knasmueller, Siegfried ; Zeiger, Errol ; Carnesoltas, Deyanira ; Cavallo, Delia ; da Silva, Juliana ; de Andrade, Vanessa M. ; Demircigil, Gonca Cakmak ; Odio, Aníbal Domínguez ; Donmez-Altuntas, Hamiyet ; Gattas, Gilka ; Giri, Ashok ; Giri, Sarbani ; Gómez-Meda, Belinda ; Gómez-Arroyo, Sandra ; Hadjidekova, Valeria ; Haveric, Anja ; Kamboj, Mala ; Kurteshi, Kemajl ; Martino-Roth, Maria Grazia ; Montoya, Regina Montero ; Nersesyan, Armen ; Pastor-Benito, Susana ; Salvadori, Daisy Maria Favero ; Shaposhnikova, Alina ; Stopper, Helga ; Thomas, Philip ; Torres-Bugarín, Olivia ; Yadav, Abhay Singh ; González, Guillermo Zúñiga ; Fenech, Michael. / The HUman MicroNucleus project on eXfoLiated buccal cells (HUMN XL) : The role of life-style, host factors, occupational exposures, health status, and assay protocol. In: Mutation Research - Reviews in Mutation Research. 2011 ; Vol. 728, No. 3. pp. 88-97.
@article{46c3b012b7f84ee7a1ea3314e421fefe,
title = "The HUman MicroNucleus project on eXfoLiated buccal cells (HUMN XL): The role of life-style, host factors, occupational exposures, health status, and assay protocol",
abstract = "The human buccal micronucleus cytome assay (BMCyt) is one of the most widely used techniques to measure genetic damage in human population studies. Reducing protocol variability, assessing the role of confounders, and estimating a range of reference values are research priorities that will be addressed by the HUMN XL collaborative study. The HUMN XL project evaluates the impact of host factors, occupation, life-style, disease status, and protocol features on the occurrence of MN in exfoliated buccal cells. In addition, the study will provide a range of reference values for all cytome endpoints. A database of 5424 subjects with buccal MN values obtained from 30 laboratories worldwide was compiled and analyzed to investigate the influence of several conditions affecting MN frequency. Random effects models were mostly used to investigate MN predictors. The estimated spontaneous MN frequency was 0.74‰ (95{\%} CI 0.52-1.05). Only staining among technical features influenced MN frequency, with an abnormal increase for non-DNA-specific stains. No effect of gender was evident, while the trend for age was highly significant (pXL project identified priorities for validation studies, increased the basic knowledge of the assay, and contributed to the creation of a laboratory network which in perspective may allow the evaluation of disease risk associated with MN frequency.",
keywords = "Assay standardization, Buccal micronucleus cytome assay (BMCyt), Exfoliated cell, Genetic instability, Molecular epidemiology, Pooled analysis",
author = "Stefano Bonassi and Erdem Coskun and Marcello Ceppi and Cecilia Lando and Claudia Bolognesi and Sema Burgaz and Nina Holland and Micheline Kirsh-Volders and Siegfried Knasmueller and Errol Zeiger and Deyanira Carnesoltas and Delia Cavallo and {da Silva}, Juliana and {de Andrade}, {Vanessa M.} and Demircigil, {Gonca Cakmak} and Odio, {An{\'i}bal Dom{\'i}nguez} and Hamiyet Donmez-Altuntas and Gilka Gattas and Ashok Giri and Sarbani Giri and Belinda G{\'o}mez-Meda and Sandra G{\'o}mez-Arroyo and Valeria Hadjidekova and Anja Haveric and Mala Kamboj and Kemajl Kurteshi and Martino-Roth, {Maria Grazia} and Montoya, {Regina Montero} and Armen Nersesyan and Susana Pastor-Benito and Salvadori, {Daisy Maria Favero} and Alina Shaposhnikova and Helga Stopper and Philip Thomas and Olivia Torres-Bugar{\'i}n and Yadav, {Abhay Singh} and Gonz{\'a}lez, {Guillermo Z{\'u}{\~n}iga} and Michael Fenech",
year = "2011",
month = "11",
doi = "10.1016/j.mrrev.2011.06.005",
language = "English",
volume = "728",
pages = "88--97",
journal = "Mutation Research - Reviews in Mutation Research",
issn = "1383-5742",
publisher = "Elsevier B.V.",
number = "3",

}

TY - JOUR

T1 - The HUman MicroNucleus project on eXfoLiated buccal cells (HUMN XL)

T2 - The role of life-style, host factors, occupational exposures, health status, and assay protocol

AU - Bonassi, Stefano

AU - Coskun, Erdem

AU - Ceppi, Marcello

AU - Lando, Cecilia

AU - Bolognesi, Claudia

AU - Burgaz, Sema

AU - Holland, Nina

AU - Kirsh-Volders, Micheline

AU - Knasmueller, Siegfried

AU - Zeiger, Errol

AU - Carnesoltas, Deyanira

AU - Cavallo, Delia

AU - da Silva, Juliana

AU - de Andrade, Vanessa M.

AU - Demircigil, Gonca Cakmak

AU - Odio, Aníbal Domínguez

AU - Donmez-Altuntas, Hamiyet

AU - Gattas, Gilka

AU - Giri, Ashok

AU - Giri, Sarbani

AU - Gómez-Meda, Belinda

AU - Gómez-Arroyo, Sandra

AU - Hadjidekova, Valeria

AU - Haveric, Anja

AU - Kamboj, Mala

AU - Kurteshi, Kemajl

AU - Martino-Roth, Maria Grazia

AU - Montoya, Regina Montero

AU - Nersesyan, Armen

AU - Pastor-Benito, Susana

AU - Salvadori, Daisy Maria Favero

AU - Shaposhnikova, Alina

AU - Stopper, Helga

AU - Thomas, Philip

AU - Torres-Bugarín, Olivia

AU - Yadav, Abhay Singh

AU - González, Guillermo Zúñiga

AU - Fenech, Michael

PY - 2011/11

Y1 - 2011/11

N2 - The human buccal micronucleus cytome assay (BMCyt) is one of the most widely used techniques to measure genetic damage in human population studies. Reducing protocol variability, assessing the role of confounders, and estimating a range of reference values are research priorities that will be addressed by the HUMN XL collaborative study. The HUMN XL project evaluates the impact of host factors, occupation, life-style, disease status, and protocol features on the occurrence of MN in exfoliated buccal cells. In addition, the study will provide a range of reference values for all cytome endpoints. A database of 5424 subjects with buccal MN values obtained from 30 laboratories worldwide was compiled and analyzed to investigate the influence of several conditions affecting MN frequency. Random effects models were mostly used to investigate MN predictors. The estimated spontaneous MN frequency was 0.74‰ (95% CI 0.52-1.05). Only staining among technical features influenced MN frequency, with an abnormal increase for non-DNA-specific stains. No effect of gender was evident, while the trend for age was highly significant (pXL project identified priorities for validation studies, increased the basic knowledge of the assay, and contributed to the creation of a laboratory network which in perspective may allow the evaluation of disease risk associated with MN frequency.

AB - The human buccal micronucleus cytome assay (BMCyt) is one of the most widely used techniques to measure genetic damage in human population studies. Reducing protocol variability, assessing the role of confounders, and estimating a range of reference values are research priorities that will be addressed by the HUMN XL collaborative study. The HUMN XL project evaluates the impact of host factors, occupation, life-style, disease status, and protocol features on the occurrence of MN in exfoliated buccal cells. In addition, the study will provide a range of reference values for all cytome endpoints. A database of 5424 subjects with buccal MN values obtained from 30 laboratories worldwide was compiled and analyzed to investigate the influence of several conditions affecting MN frequency. Random effects models were mostly used to investigate MN predictors. The estimated spontaneous MN frequency was 0.74‰ (95% CI 0.52-1.05). Only staining among technical features influenced MN frequency, with an abnormal increase for non-DNA-specific stains. No effect of gender was evident, while the trend for age was highly significant (pXL project identified priorities for validation studies, increased the basic knowledge of the assay, and contributed to the creation of a laboratory network which in perspective may allow the evaluation of disease risk associated with MN frequency.

KW - Assay standardization

KW - Buccal micronucleus cytome assay (BMCyt)

KW - Exfoliated cell

KW - Genetic instability

KW - Molecular epidemiology

KW - Pooled analysis

UR - http://www.scopus.com/inward/record.url?scp=80053592718&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80053592718&partnerID=8YFLogxK

U2 - 10.1016/j.mrrev.2011.06.005

DO - 10.1016/j.mrrev.2011.06.005

M3 - Article

C2 - 21763453

AN - SCOPUS:80053592718

VL - 728

SP - 88

EP - 97

JO - Mutation Research - Reviews in Mutation Research

JF - Mutation Research - Reviews in Mutation Research

SN - 1383-5742

IS - 3

ER -