The human muscle proteome in aging

Cecilia Gelfi, Agnese Viganò, Marilena Ripamonti, Alessandro Pontoglio, Shajna Begum, Maria Antonietta Pellegrino, Bruno Grassi, Roberto Bottinelli, Robin Wait, Paolo Cerretelli

Research output: Contribution to journalArticle

Abstract

The aim of the present study was to assess age-dependent changes of proteins in the vastus lateralis muscle of physically active elderly and young subjects by a combination of two-dimensional difference gel electrophoresis, SDS-PAGE and ESI-MS/MS. The differences observed in the elderly group included down-regulation of regulatory myosin light chains, particularly the phosphorylated isoforms, a higher proportion of myosin heavy chain isoforms 1 and 2A, and enhanced oxidative and reduced glycolytic capacity.

Original languageEnglish
Pages (from-to)1344-1353
Number of pages10
JournalJournal of Proteome Research
Volume5
Issue number6
DOIs
Publication statusPublished - Jun 2006

Keywords

  • 2-D DIGE
  • Aging
  • Mass spectrometry
  • Myosin
  • Phosphorylation

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry

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  • Cite this

    Gelfi, C., Viganò, A., Ripamonti, M., Pontoglio, A., Begum, S., Pellegrino, M. A., Grassi, B., Bottinelli, R., Wait, R., & Cerretelli, P. (2006). The human muscle proteome in aging. Journal of Proteome Research, 5(6), 1344-1353. https://doi.org/10.1021/pr050414x