The human RNASET2 protein affects the polarization pattern of human macrophages in vitro

Debora Scaldaferri, Annalisa Bosi, Marco Fabbri, Edoardo Pedrini, Antonio Inforzato, Roberto Valli, Annalisa Frattini, Annarosaria De Vito, Douglas M Noonan, Roberto Taramelli, Lorenzo Mortara, Francesco Acquati

Research output: Contribution to journalArticle

Abstract

Macrophages represent key inflammatory cellular effectors of the innate immune response. Despite being widely acknowledged as professional phagocytes, the functional roles played by these cells have been progressively widened over the years to encompass regulation of the adaptive immune system, stimulation or suppression of cancer cell growth and tissue remodeling. These diverse functional features have led to the concept of "macrophage plasticity", i.e. the ability of these cells to express a wide range of phenotypes endowed with different functional roles. Several activation programs have been described for mammalian macrophages, based mainly on their differential transcriptional profiles. Based on established in vitro experimental conditions, many researchers currently refer to the M1 (or M1-like) and M2 (or M2-like) terms to describe the two extremes of a rather broad spectrum of polarization states that macrophages can experience in vivo. In light of the widely recognized opposite roles of M1-like and M2-like macrophages on cancer growth, and our largely incomplete knowledge of the cellular and molecular mechanisms underlying the establishment of the M1-like versus M2-like balance within a tumor mass, we report here results from in vitro assays pointing at the human RNASET2 gene as a potential regulator of the balance between M1-like/M2-like macrophage polarization. Not only do our results confirm previous in vivo data, thus further supporting a role for this pleiotropic protein in the innate immune system, but they also define RNASET2 as a new molecular target with potential applications for in vivo reprogramming of macrophage polarization, an increasingly appraised anticancer strategy.

Original languageEnglish
Pages (from-to)102-111
Number of pages10
JournalImmunology Letters
Volume203
DOIs
Publication statusPublished - Nov 2018

Fingerprint

Macrophages
Immune System
Neoplasms
Phagocytes
Growth
In Vitro Techniques
human RNASET2 protein
Innate Immunity
Research Personnel
Phenotype
Genes
Proteins

Cite this

The human RNASET2 protein affects the polarization pattern of human macrophages in vitro. / Scaldaferri, Debora; Bosi, Annalisa; Fabbri, Marco; Pedrini, Edoardo; Inforzato, Antonio; Valli, Roberto; Frattini, Annalisa; De Vito, Annarosaria; Noonan, Douglas M; Taramelli, Roberto; Mortara, Lorenzo; Acquati, Francesco.

In: Immunology Letters, Vol. 203, 11.2018, p. 102-111.

Research output: Contribution to journalArticle

Scaldaferri, D, Bosi, A, Fabbri, M, Pedrini, E, Inforzato, A, Valli, R, Frattini, A, De Vito, A, Noonan, DM, Taramelli, R, Mortara, L & Acquati, F 2018, 'The human RNASET2 protein affects the polarization pattern of human macrophages in vitro', Immunology Letters, vol. 203, pp. 102-111. https://doi.org/10.1016/j.imlet.2018.09.005
Scaldaferri, Debora ; Bosi, Annalisa ; Fabbri, Marco ; Pedrini, Edoardo ; Inforzato, Antonio ; Valli, Roberto ; Frattini, Annalisa ; De Vito, Annarosaria ; Noonan, Douglas M ; Taramelli, Roberto ; Mortara, Lorenzo ; Acquati, Francesco. / The human RNASET2 protein affects the polarization pattern of human macrophages in vitro. In: Immunology Letters. 2018 ; Vol. 203. pp. 102-111.
@article{0a00ecbdcc1945e7b3c7db3504672644,
title = "The human RNASET2 protein affects the polarization pattern of human macrophages in vitro",
abstract = "Macrophages represent key inflammatory cellular effectors of the innate immune response. Despite being widely acknowledged as professional phagocytes, the functional roles played by these cells have been progressively widened over the years to encompass regulation of the adaptive immune system, stimulation or suppression of cancer cell growth and tissue remodeling. These diverse functional features have led to the concept of {"}macrophage plasticity{"}, i.e. the ability of these cells to express a wide range of phenotypes endowed with different functional roles. Several activation programs have been described for mammalian macrophages, based mainly on their differential transcriptional profiles. Based on established in vitro experimental conditions, many researchers currently refer to the M1 (or M1-like) and M2 (or M2-like) terms to describe the two extremes of a rather broad spectrum of polarization states that macrophages can experience in vivo. In light of the widely recognized opposite roles of M1-like and M2-like macrophages on cancer growth, and our largely incomplete knowledge of the cellular and molecular mechanisms underlying the establishment of the M1-like versus M2-like balance within a tumor mass, we report here results from in vitro assays pointing at the human RNASET2 gene as a potential regulator of the balance between M1-like/M2-like macrophage polarization. Not only do our results confirm previous in vivo data, thus further supporting a role for this pleiotropic protein in the innate immune system, but they also define RNASET2 as a new molecular target with potential applications for in vivo reprogramming of macrophage polarization, an increasingly appraised anticancer strategy.",
author = "Debora Scaldaferri and Annalisa Bosi and Marco Fabbri and Edoardo Pedrini and Antonio Inforzato and Roberto Valli and Annalisa Frattini and {De Vito}, Annarosaria and Noonan, {Douglas M} and Roberto Taramelli and Lorenzo Mortara and Francesco Acquati",
note = "Copyright {\circledC} 2018 European Federation of Immunological Societies. Published by Elsevier B.V. All rights reserved.",
year = "2018",
month = "11",
doi = "10.1016/j.imlet.2018.09.005",
language = "English",
volume = "203",
pages = "102--111",
journal = "Immunology Letters",
issn = "0165-2478",
publisher = "Elsevier",

}

TY - JOUR

T1 - The human RNASET2 protein affects the polarization pattern of human macrophages in vitro

AU - Scaldaferri, Debora

AU - Bosi, Annalisa

AU - Fabbri, Marco

AU - Pedrini, Edoardo

AU - Inforzato, Antonio

AU - Valli, Roberto

AU - Frattini, Annalisa

AU - De Vito, Annarosaria

AU - Noonan, Douglas M

AU - Taramelli, Roberto

AU - Mortara, Lorenzo

AU - Acquati, Francesco

N1 - Copyright © 2018 European Federation of Immunological Societies. Published by Elsevier B.V. All rights reserved.

PY - 2018/11

Y1 - 2018/11

N2 - Macrophages represent key inflammatory cellular effectors of the innate immune response. Despite being widely acknowledged as professional phagocytes, the functional roles played by these cells have been progressively widened over the years to encompass regulation of the adaptive immune system, stimulation or suppression of cancer cell growth and tissue remodeling. These diverse functional features have led to the concept of "macrophage plasticity", i.e. the ability of these cells to express a wide range of phenotypes endowed with different functional roles. Several activation programs have been described for mammalian macrophages, based mainly on their differential transcriptional profiles. Based on established in vitro experimental conditions, many researchers currently refer to the M1 (or M1-like) and M2 (or M2-like) terms to describe the two extremes of a rather broad spectrum of polarization states that macrophages can experience in vivo. In light of the widely recognized opposite roles of M1-like and M2-like macrophages on cancer growth, and our largely incomplete knowledge of the cellular and molecular mechanisms underlying the establishment of the M1-like versus M2-like balance within a tumor mass, we report here results from in vitro assays pointing at the human RNASET2 gene as a potential regulator of the balance between M1-like/M2-like macrophage polarization. Not only do our results confirm previous in vivo data, thus further supporting a role for this pleiotropic protein in the innate immune system, but they also define RNASET2 as a new molecular target with potential applications for in vivo reprogramming of macrophage polarization, an increasingly appraised anticancer strategy.

AB - Macrophages represent key inflammatory cellular effectors of the innate immune response. Despite being widely acknowledged as professional phagocytes, the functional roles played by these cells have been progressively widened over the years to encompass regulation of the adaptive immune system, stimulation or suppression of cancer cell growth and tissue remodeling. These diverse functional features have led to the concept of "macrophage plasticity", i.e. the ability of these cells to express a wide range of phenotypes endowed with different functional roles. Several activation programs have been described for mammalian macrophages, based mainly on their differential transcriptional profiles. Based on established in vitro experimental conditions, many researchers currently refer to the M1 (or M1-like) and M2 (or M2-like) terms to describe the two extremes of a rather broad spectrum of polarization states that macrophages can experience in vivo. In light of the widely recognized opposite roles of M1-like and M2-like macrophages on cancer growth, and our largely incomplete knowledge of the cellular and molecular mechanisms underlying the establishment of the M1-like versus M2-like balance within a tumor mass, we report here results from in vitro assays pointing at the human RNASET2 gene as a potential regulator of the balance between M1-like/M2-like macrophage polarization. Not only do our results confirm previous in vivo data, thus further supporting a role for this pleiotropic protein in the innate immune system, but they also define RNASET2 as a new molecular target with potential applications for in vivo reprogramming of macrophage polarization, an increasingly appraised anticancer strategy.

U2 - 10.1016/j.imlet.2018.09.005

DO - 10.1016/j.imlet.2018.09.005

M3 - Article

C2 - 30218741

VL - 203

SP - 102

EP - 111

JO - Immunology Letters

JF - Immunology Letters

SN - 0165-2478

ER -