Abstract
Recent studies using cloned antigen-specific T lymphocytes and monoclonal antibodies directed at their various surface glycoprotein components have led to the identification of the human T-cell antigen receptor as a surface complex comprised of a clonotypic 90-kD Ti heterodimer and the invariant 20- and 25-kD T3 molecules. Approximately 30,000-40,000 Ti and T3 molecules exist on the surface of human T lymphocytes. These glycoproteins are acquired and expressed during late thymic ontogeny, thus providing the structural basis for immunologic competence. The α and β subunits of Ti bear no precursor-product relationship to one another and are encoded by separate genes. Moreover, the presence of unique peptides following proteolysis of different Ti molecules isolated by non-cross-reactive anticlonotypic monoclonal antibodies supports the notion that variable regions exist within both the α and the β subunits. N-Terminal amino acid sequencing and molecular cloning of the Ti β subunit further show that it bears an homology to the first V-region framework of immunoglobulin light chains and represents the product of a gene that rearranges specifically in T lymphocytes. Triggering of the T3-Ti receptor complex gives rise to specific antigen-induced proliferation through an autocrine pathway involving endogenous IL-2 production, release, and subsequent binding to IL-2 receptors. The implications of these findings for understanding human T-cell growth and its regulation in disease states are discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 141-157 |
| Number of pages | 17 |
| Journal | Journal of Clinical Immunology |
| Volume | 5 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - May 1985 |
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Keywords
- gene
- T cell antigen receptor
- T cell subset
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
Cite this
The human T-cell receptor. / Acuto, Oreste; Fabbi, Marina; Bensussan, Armand; Milanese, Claudio; Campen, Thomas J.; Royer, Hans Dieter; Reinherz, Ellis L.
In: Journal of Clinical Immunology, Vol. 5, No. 3, 05.1985, p. 141-157.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The human T-cell receptor
AU - Acuto, Oreste
AU - Fabbi, Marina
AU - Bensussan, Armand
AU - Milanese, Claudio
AU - Campen, Thomas J.
AU - Royer, Hans Dieter
AU - Reinherz, Ellis L.
PY - 1985/5
Y1 - 1985/5
N2 - Recent studies using cloned antigen-specific T lymphocytes and monoclonal antibodies directed at their various surface glycoprotein components have led to the identification of the human T-cell antigen receptor as a surface complex comprised of a clonotypic 90-kD Ti heterodimer and the invariant 20- and 25-kD T3 molecules. Approximately 30,000-40,000 Ti and T3 molecules exist on the surface of human T lymphocytes. These glycoproteins are acquired and expressed during late thymic ontogeny, thus providing the structural basis for immunologic competence. The α and β subunits of Ti bear no precursor-product relationship to one another and are encoded by separate genes. Moreover, the presence of unique peptides following proteolysis of different Ti molecules isolated by non-cross-reactive anticlonotypic monoclonal antibodies supports the notion that variable regions exist within both the α and the β subunits. N-Terminal amino acid sequencing and molecular cloning of the Ti β subunit further show that it bears an homology to the first V-region framework of immunoglobulin light chains and represents the product of a gene that rearranges specifically in T lymphocytes. Triggering of the T3-Ti receptor complex gives rise to specific antigen-induced proliferation through an autocrine pathway involving endogenous IL-2 production, release, and subsequent binding to IL-2 receptors. The implications of these findings for understanding human T-cell growth and its regulation in disease states are discussed.
AB - Recent studies using cloned antigen-specific T lymphocytes and monoclonal antibodies directed at their various surface glycoprotein components have led to the identification of the human T-cell antigen receptor as a surface complex comprised of a clonotypic 90-kD Ti heterodimer and the invariant 20- and 25-kD T3 molecules. Approximately 30,000-40,000 Ti and T3 molecules exist on the surface of human T lymphocytes. These glycoproteins are acquired and expressed during late thymic ontogeny, thus providing the structural basis for immunologic competence. The α and β subunits of Ti bear no precursor-product relationship to one another and are encoded by separate genes. Moreover, the presence of unique peptides following proteolysis of different Ti molecules isolated by non-cross-reactive anticlonotypic monoclonal antibodies supports the notion that variable regions exist within both the α and the β subunits. N-Terminal amino acid sequencing and molecular cloning of the Ti β subunit further show that it bears an homology to the first V-region framework of immunoglobulin light chains and represents the product of a gene that rearranges specifically in T lymphocytes. Triggering of the T3-Ti receptor complex gives rise to specific antigen-induced proliferation through an autocrine pathway involving endogenous IL-2 production, release, and subsequent binding to IL-2 receptors. The implications of these findings for understanding human T-cell growth and its regulation in disease states are discussed.
KW - gene
KW - T cell antigen receptor
KW - T cell subset
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UR - http://www.scopus.com/inward/citedby.url?scp=0021920340&partnerID=8YFLogxK
U2 - 10.1007/BF00915505
DO - 10.1007/BF00915505
M3 - Article
C2 - 2409103
AN - SCOPUS:0021920340
VL - 5
SP - 141
EP - 157
JO - Journal of Clinical Immunology
JF - Journal of Clinical Immunology
SN - 0271-9142
IS - 3
ER -