The human transaldolase gene (TALDO1) is located on chromosome 11 at p15.4-p15.5

Katalin Banki, Roger L. Eddy, Thomas B. Shows, David L. Halladay, Florencia Bullrich, Carlo M. Croce, Vesna Jurecic, Antonio Baldini, Andras Perl

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Abstract

Transaldolase (TAL) is a key enzyme of the pentose phosphate pathway, which is responsible for generation of reducing equivalents to protect cellular integrity from reactive oxygen intermediates. While exons 2 and 3 are highly repetitive, the complete TAL-H gene is mapped to a single genomic locus (TALDO12) by several independent approaches. Southern blot hybridization of a 827-bp 3' EcoRI fragment of the TAL-H cDNA to human-mouse somatic cell hybrid DNA localized TALDO1 to the p13 → pter region of chromosome 11. Fluorescence in situ hybridization with a 15-kb genomic fragment harboring exons 1 and 2 mapped TALDO1 to 11p15.4-p15.5. A truncated and mutated segment of TAL-H exon 5 terminating with a poly(A) tail was identified in a pseudogene locus (TALDOP1) on chromosome 1. Reverse transcriptase-PCR studies of human-mouse somatic cell hybrids revealed the presence of the functional TAL-H gene on chromosome 11 and its absence on human chromosome 1. Mapping of radiation hybrids placed TALDO1 between markers WI-1421 and D11S922 on 11p15.

Original languageEnglish
Pages (from-to)233-238
Number of pages6
JournalGenomics
Volume45
Issue number1
DOIs
Publication statusPublished - Oct 1 1997

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ASJC Scopus subject areas

  • Genetics

Cite this

Banki, K., Eddy, R. L., Shows, T. B., Halladay, D. L., Bullrich, F., Croce, C. M., Jurecic, V., Baldini, A., & Perl, A. (1997). The human transaldolase gene (TALDO1) is located on chromosome 11 at p15.4-p15.5. Genomics, 45(1), 233-238. https://doi.org/10.1006/geno.1997.4932