The human type VI collagen gene. mRNA and protein variants of the α3 chain generated by alternative splicing of an additional 5-end exon

S. Zanussi, R. Doliana, D. Segat, P. Bonaldo, A. Colombatti

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The amino- and carboxyl-terminal globular domains of type VI collagen are composed of several homologous modules similar to the type A collagen- binding modules present in von Willebrand factor. The human α3(VI) chain that contributes most of the amino-terminal globule appears heterogeneous in size as a result of alternative splicing of two exons (Stokes D. G., Saitta, B., Timpl, R., and Chu, M.-L. (1991) J. Biol. Chem. 266, 8626-8633). In the present study, we report a further characterization of the 5'-end of the gene of the human α3(VI) chain and show that transcription initiates at multiple sites. Southern blotting and DNA sequencing indicate that there is an additional type A exon (A9/N10) at about 1.8 kilobase pairs downstream of the exon coding for the signal peptide. The open reading frame of this additional exon reveals 1 cysteine and three potential N-glycosylation sites. Polymerase chain reaction, Northern blotting, and RNase protection assays demonstrate that exon A9/N10 is subject to alternative splicing in normal and tumor cell lines and that this generates more protein variants of the α3(VI) chain than expected before. A comparison with the corresponding amino-terminal globule of the chicken α3(VI) chain shows the presence of 1 additional cysteine in this portion of the molecule and suggests that human type VI collagen has more possibilities for structural and functional variations compared to chicken type VI collagen.

Original languageEnglish
Pages (from-to)24082-24089
Number of pages8
JournalJournal of Biological Chemistry
Volume267
Issue number33
Publication statusPublished - 1992

Fingerprint

Collagen Type III
Alternative Splicing
Exons
Collagen
Genes
Messenger RNA
Proteins
Cysteine
Chickens
Glycosylation
Polymerase chain reaction
von Willebrand Factor
Transcription
Ribonucleases
Protein Sorting Signals
Southern Blotting
Tumor Cell Line
DNA Sequence Analysis
Northern Blotting
Open Reading Frames

ASJC Scopus subject areas

  • Biochemistry

Cite this

The human type VI collagen gene. mRNA and protein variants of the α3 chain generated by alternative splicing of an additional 5-end exon. / Zanussi, S.; Doliana, R.; Segat, D.; Bonaldo, P.; Colombatti, A.

In: Journal of Biological Chemistry, Vol. 267, No. 33, 1992, p. 24082-24089.

Research output: Contribution to journalArticle

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T1 - The human type VI collagen gene. mRNA and protein variants of the α3 chain generated by alternative splicing of an additional 5-end exon

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AU - Doliana, R.

AU - Segat, D.

AU - Bonaldo, P.

AU - Colombatti, A.

PY - 1992

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