The human urinary exosome as a potential metabolic effector cargo

Maurizio Bruschi, Silvia Ravera, Laura Santucci, Giovanni Candiano, Martina Bartolucci, Daniela Calzia, Chiara Lavarello, Elvira Inglese, Andrea Petretto, Gianmarco Ghiggeri, Isabella Panfoli

Research output: Contribution to journalArticle

Abstract

Exosomes are nanovesicles, derived from the endocytic pathway, released by most cell types and found in many body fluids, including urine. A variety of exosomal functions have been reported, including transfer of RNA, cell communication, control of apoptosis and protein lifespan. Exosomes from mesenchymal stem cells can rescue bioenergetics of injured cells. Here the urinary exosome proteome, non-urinary exosome proteome and urinome are compared. A consistent number of identified proteins cluster to metabolic functions. Cytoscape software analysis based on biological processes gene ontology database shows that metabolic pathways such as aerobic glycolysis and oxidative phosphorylation have a high probability (p ≤ 0.05) of being expressed and therefore functional. A metabolic function appears to be associated with human urinary exosomes, whose relevance experimental studies can assess.

Original languageEnglish
Pages (from-to)425-432
Number of pages8
JournalExpert Review of Proteomics
Volume12
Issue number4
DOIs
Publication statusPublished - Aug 1 2015

Keywords

  • exosome
  • FoF1-ATP synthase
  • mass spectrometry
  • oxidative phosphorylation
  • proteomics
  • respiratory complexes
  • urinome

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

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