The HUMNxl scoring criteria for different cell types and nuclear anomalies in the buccal micronucleus cytome assay - An update and expanded photogallery

Claudia Bolognesi, Siegfried Knasmueller, Armen Nersesyan, Philip Thomas, Michael Fenech

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

The buccal micronucleus cytome assay is a minimally invasive cytological and interphase cytogenetic technique for measuring DNA damage and cell death biomarkers in the oral epithelium. In this report we provide an updated and more comprehensive version of the cellular and nuclear scoring criteria used in the assay accompanied with a photogallery of the various cell types and nuclear anomalies. These detailed scoring criteria complement previous published protocols of this assay and form the basis for guiding intra- and inter-laboratory slide scoring comparisons. The scoring criteria update described in this paper is the outcome of ongoing efforts of the HUMN and HUMNxl projects (www.humn.org) to standardize the application of micronucleus assay for use in human biomonitoring and to update procedures as knowledge on mechanisms and technical capability improvements.

Original languageEnglish
Pages (from-to)100-113
Number of pages14
JournalMutation Research - Reviews in Mutation Research
Volume753
Issue number2
DOIs
Publication statusPublished - Oct 2013

Fingerprint

Micronucleus Tests
Cheek
Environmental Monitoring
Cytogenetic Analysis
Interphase
DNA Damage
Cell Death
Epithelium
Biomarkers

Keywords

  • Biomonitoring
  • Buccal micronucleus cytome assay (BMcyt)
  • DNA damage
  • Exfoliated cells

ASJC Scopus subject areas

  • Genetics
  • Health, Toxicology and Mutagenesis
  • Molecular Biology
  • Medicine(all)

Cite this

The HUMNxl scoring criteria for different cell types and nuclear anomalies in the buccal micronucleus cytome assay - An update and expanded photogallery. / Bolognesi, Claudia; Knasmueller, Siegfried; Nersesyan, Armen; Thomas, Philip; Fenech, Michael.

In: Mutation Research - Reviews in Mutation Research, Vol. 753, No. 2, 10.2013, p. 100-113.

Research output: Contribution to journalArticle

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