Abstract
The buccal micronucleus cytome assay is a minimally invasive cytological and interphase cytogenetic technique for measuring DNA damage and cell death biomarkers in the oral epithelium. In this report we provide an updated and more comprehensive version of the cellular and nuclear scoring criteria used in the assay accompanied with a photogallery of the various cell types and nuclear anomalies. These detailed scoring criteria complement previous published protocols of this assay and form the basis for guiding intra- and inter-laboratory slide scoring comparisons. The scoring criteria update described in this paper is the outcome of ongoing efforts of the HUMN and HUMNxl projects (www.humn.org) to standardize the application of micronucleus assay for use in human biomonitoring and to update procedures as knowledge on mechanisms and technical capability improvements.
| Original language | English |
|---|---|
| Pages (from-to) | 100-113 |
| Number of pages | 14 |
| Journal | Mutation Research - Reviews in Mutation Research |
| Volume | 753 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Oct 2013 |
Fingerprint
Keywords
- Biomonitoring
- Buccal micronucleus cytome assay (BMcyt)
- DNA damage
- Exfoliated cells
ASJC Scopus subject areas
- Genetics
- Health, Toxicology and Mutagenesis
- Molecular Biology
- Medicine(all)
Cite this
The HUMNxl scoring criteria for different cell types and nuclear anomalies in the buccal micronucleus cytome assay - An update and expanded photogallery. / Bolognesi, Claudia; Knasmueller, Siegfried; Nersesyan, Armen; Thomas, Philip; Fenech, Michael.
In: Mutation Research - Reviews in Mutation Research, Vol. 753, No. 2, 10.2013, p. 100-113.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The HUMNxl scoring criteria for different cell types and nuclear anomalies in the buccal micronucleus cytome assay - An update and expanded photogallery
AU - Bolognesi, Claudia
AU - Knasmueller, Siegfried
AU - Nersesyan, Armen
AU - Thomas, Philip
AU - Fenech, Michael
PY - 2013/10
Y1 - 2013/10
N2 - The buccal micronucleus cytome assay is a minimally invasive cytological and interphase cytogenetic technique for measuring DNA damage and cell death biomarkers in the oral epithelium. In this report we provide an updated and more comprehensive version of the cellular and nuclear scoring criteria used in the assay accompanied with a photogallery of the various cell types and nuclear anomalies. These detailed scoring criteria complement previous published protocols of this assay and form the basis for guiding intra- and inter-laboratory slide scoring comparisons. The scoring criteria update described in this paper is the outcome of ongoing efforts of the HUMN and HUMNxl projects (www.humn.org) to standardize the application of micronucleus assay for use in human biomonitoring and to update procedures as knowledge on mechanisms and technical capability improvements.
AB - The buccal micronucleus cytome assay is a minimally invasive cytological and interphase cytogenetic technique for measuring DNA damage and cell death biomarkers in the oral epithelium. In this report we provide an updated and more comprehensive version of the cellular and nuclear scoring criteria used in the assay accompanied with a photogallery of the various cell types and nuclear anomalies. These detailed scoring criteria complement previous published protocols of this assay and form the basis for guiding intra- and inter-laboratory slide scoring comparisons. The scoring criteria update described in this paper is the outcome of ongoing efforts of the HUMN and HUMNxl projects (www.humn.org) to standardize the application of micronucleus assay for use in human biomonitoring and to update procedures as knowledge on mechanisms and technical capability improvements.
KW - Biomonitoring
KW - Buccal micronucleus cytome assay (BMcyt)
KW - DNA damage
KW - Exfoliated cells
UR - http://www.scopus.com/inward/record.url?scp=84887015984&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84887015984&partnerID=8YFLogxK
U2 - 10.1016/j.mrrev.2013.07.002
DO - 10.1016/j.mrrev.2013.07.002
M3 - Article
C2 - 23942275
AN - SCOPUS:84887015984
VL - 753
SP - 100
EP - 113
JO - Mutation Research - Reviews in Mutation Research
JF - Mutation Research - Reviews in Mutation Research
SN - 1383-5742
IS - 2
ER -