The catalytic properties of human urokinase have been investigated using a synthetic chromogenic substrate; α-CBZ-l-lysine-p-nitrophenyl ester (ZLNP). The enzymatic assay based on the rate of hydrolysis of ZLNP offers several advantages over other methods currently employed in different laboratories. The steady state parameters of the two purified forms of human urokinase, which differ in molecular weight (33,000 and 54,000 daltons), have been determined over the pH range 5.2-7.8, and found to be indistinguishable.
ASJC Scopus subject areas
- Molecular Biology