The IL-2 receptor present on human embryonic fibroblasts is functional in the absence of P64/IL-2Rγ chain

S. Plaisance, E. Rubinstein, A. Allileche, P. Benoit, C. Jasmin, B. Azzarone

Research output: Contribution to journalArticle

Abstract

In this study, we have investigated the IL-2Rγ gene expression in several human embryonic fibroblasts which express other components of the IL-2 receptor (IL-2R). Polymerase chain reaction did not allow us to detect IL-2Rγ transcripts in these cells but the functionality of the IL-2Rαβ was not affected. Indeed, in the human IL-2Rα+β+γ- embryonic lung fibroblasts ICIG-7, IL-2 induced identical phenomena to those previously reported in lymphoid cells: rapid internalization of the IL-2Rαβ - IL-2 complex, specific phosphorylation of cellular proteins (56 and 38 kDa) and up-regulation of ICAM-1 expression. IL-2 induction of ICAM-1 was only observed in sparse cultures and for IL-2 concentrations over 180 pM. We have also observed, in these fibroblastic cells, the up-regulation of ICAM-2 expression by IL-2, both in sparse and dense cultures. These data show that p64/1L-2Rγ expression in human embryonic fibroblasts does not correlate with the ability of the IL-2R to deliver a biological signal.

Original languageEnglish
Pages (from-to)843-848
Number of pages6
JournalInternational Immunology
Volume5
Issue number8
Publication statusPublished - 1993

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Keywords

  • γ chain
  • ICAM-1
  • ICAM-2
  • IL-2R

ASJC Scopus subject areas

  • Immunology
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Public Health, Environmental and Occupational Health
  • Neuropsychology and Physiological Psychology
  • Transplantation

Cite this

Plaisance, S., Rubinstein, E., Allileche, A., Benoit, P., Jasmin, C., & Azzarone, B. (1993). The IL-2 receptor present on human embryonic fibroblasts is functional in the absence of P64/IL-2Rγ chain. International Immunology, 5(8), 843-848.