Abstract
In this study, we have investigated the IL-2Rγ gene expression in several human embryonic fibroblasts which express other components of the IL-2 receptor (IL-2R). Polymerase chain reaction did not allow us to detect IL-2Rγ transcripts in these cells but the functionality of the IL-2Rαβ was not affected. Indeed, in the human IL-2Rα+β+γ- embryonic lung fibroblasts ICIG-7, IL-2 induced identical phenomena to those previously reported in lymphoid cells: rapid internalization of the IL-2Rαβ - IL-2 complex, specific phosphorylation of cellular proteins (56 and 38 kDa) and up-regulation of ICAM-1 expression. IL-2 induction of ICAM-1 was only observed in sparse cultures and for IL-2 concentrations over 180 pM. We have also observed, in these fibroblastic cells, the up-regulation of ICAM-2 expression by IL-2, both in sparse and dense cultures. These data show that p64/1L-2Rγ expression in human embryonic fibroblasts does not correlate with the ability of the IL-2R to deliver a biological signal.
Original language | English |
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Pages (from-to) | 843-848 |
Number of pages | 6 |
Journal | International Immunology |
Volume | 5 |
Issue number | 8 |
Publication status | Published - 1993 |
Keywords
- γ chain
- ICAM-1
- ICAM-2
- IL-2R
ASJC Scopus subject areas
- Immunology
- Statistics, Probability and Uncertainty
- Applied Mathematics
- Public Health, Environmental and Occupational Health
- Neuropsychology and Physiological Psychology
- Transplantation