The immune inhibitory receptor LAIR-1 is highly expressed by plasmacytoid dendritic cells and acts complementary with NKp44 to control IFNα production

Irene Bonaccorsi, Claudia Cantoni, Paolo Carrega, Daniela Oliveri, Gabrielle Lui, Romana Conte, Michele Navarra, Riccardo Cavaliere, Elisabetta Traggiai, Marco Gattorno, Alberto Martini, Maria Cristina Mingari, Alessandro Moretta, Guido Ferlazzo

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Plasmacytoid dendritic cells (pDCs) are a subset of dendritic cells endowed with the capacity of producing large amounts of IFNa. Here we show that the Leukocyte-Associated Ig-like Receptor-1 (LAIR-1) is abundantly expressed on pDCs (the highest expression among all leukocytes) and its cross-linking inhibits IFNa production in response to Toll-like receptor ligands. Remarkably, LAIR-1 expression in pDCs is down-regulated in the presence of interleukin (IL)-3, thus indicating coordinated functions with NKp44, another pDC inhibitory receptor, which is conversely induced by IL-3. Nevertheless, the expression of NKp44 in pDCs isolated from secondary lymphoid organs, which is thought to be influenced by IL-3, is not coupled to a decreased expression of LAIR-1. Interestingly, pDCs isolated from peripheral blood of systemic lupus erithematosus (SLE) patients express lower levels of LAIR-1 while displaying slight but consistent expression of NKp44, usually undetectable on pDCs derived from healthy donors. Using sera derived from SLE patients, we show that LAIR-1 and NKp44 display synergistic inhibitory effects on IFNa production by interleukin IL-3 cultured pDCs stimulated with DNA immunocomplexes. In conclusion, our results indicate that the inhibitory function of LAIR-1 may play a relevant role in the mechanisms controlling IFNa production by pDCs both in normal and pathological innate immune responses.

Original languageEnglish
Article numbere15080
JournalPLoS One
Volume5
Issue number11
DOIs
Publication statusPublished - 2010

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Production control
dendritic cells
Dendritic Cells
leukocytes
Leukocytes
interleukin-3
receptors
Interleukin-3
Natural Cytotoxicity Triggering Receptor 2
Toll-Like Receptors
Interleukins
interleukins
blood serum
crosslinking
Innate Immunity
cultured cells
Blood
Tissue Donors
Ligands
DNA

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

The immune inhibitory receptor LAIR-1 is highly expressed by plasmacytoid dendritic cells and acts complementary with NKp44 to control IFNα production. / Bonaccorsi, Irene; Cantoni, Claudia; Carrega, Paolo; Oliveri, Daniela; Lui, Gabrielle; Conte, Romana; Navarra, Michele; Cavaliere, Riccardo; Traggiai, Elisabetta; Gattorno, Marco; Martini, Alberto; Mingari, Maria Cristina; Moretta, Alessandro; Ferlazzo, Guido.

In: PLoS One, Vol. 5, No. 11, e15080, 2010.

Research output: Contribution to journalArticle

Bonaccorsi, Irene ; Cantoni, Claudia ; Carrega, Paolo ; Oliveri, Daniela ; Lui, Gabrielle ; Conte, Romana ; Navarra, Michele ; Cavaliere, Riccardo ; Traggiai, Elisabetta ; Gattorno, Marco ; Martini, Alberto ; Mingari, Maria Cristina ; Moretta, Alessandro ; Ferlazzo, Guido. / The immune inhibitory receptor LAIR-1 is highly expressed by plasmacytoid dendritic cells and acts complementary with NKp44 to control IFNα production. In: PLoS One. 2010 ; Vol. 5, No. 11.
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AU - Oliveri, Daniela

AU - Lui, Gabrielle

AU - Conte, Romana

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