The ImmunoCAP ISAC molecular allergology approach in adult multi-sensitized Italian patients with respiratory symptoms

Giovanni Melioli, Floriano Bonifazi, Sergio Bonini, Enrico Maggi, Michele Mussap, Gianni Passalacqua, Enzo Renato Rossi, Angelo Vacca, Giorgio Walter Canonica

Research output: Contribution to journalArticle

Abstract

Objectives: To evaluate the performances of an allergen microarray in multi-sensitized allergic patients with respiratory symptoms. Design and methods: 321 patients and 92 controls were included in this study. Specific serum IgE were assayed using ImmunoCAP ISAC, a microarray containing 103 components derived from 47 allergens and results were compared with extract-based ImmunoCAP Allergens sIgE to 15 common airborne allergens. Results: The reproducibility of ISAC was good. The Positive Percent Agreement (PPA) varied between 75% and 100% for sIgE levels above 1 kUA/l. For samples with sIgE levels below 0.1. kUA/l, the Negative Percent Agreement (NPA) ranged between 90% and 100%. Notably, 58% of respiratory allergy patients had IgE to food-specific proteins and 52% resulted sensitized to cross-reactive pan-allergens. Conclusion: ImmunoCAP ISAC detects allergen sensitization at component level and adds important information by defining both cross and co-sensitization to a large variety of allergen molecules.

Original languageEnglish
Pages (from-to)1005-1011
Number of pages7
JournalClinical Biochemistry
Volume44
Issue number12
DOIs
Publication statusPublished - Aug 2011

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Keywords

  • Allergens
  • Component resolved diagnostics
  • Microarray
  • Molecular allergology
  • SIgE

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Melioli, G., Bonifazi, F., Bonini, S., Maggi, E., Mussap, M., Passalacqua, G., Rossi, E. R., Vacca, A., & Canonica, G. W. (2011). The ImmunoCAP ISAC molecular allergology approach in adult multi-sensitized Italian patients with respiratory symptoms. Clinical Biochemistry, 44(12), 1005-1011. https://doi.org/10.1016/j.clinbiochem.2011.05.007