The immunological effects of extracorporeal photopheresis unraveled: Induction of tolerogenic dendritic cells in vitro and regulatory T cells in vivo

Andrea Lamioni, Francesco Parisi, Giancarlo Isacchi, Ezio Giorda, Silvia Di Cesare, Attilio Landolfo, Francesco Cenci, Gian Franco Bottazzo, Rita Carsetti

Research output: Contribution to journalArticle

Abstract

Extracorporeal photopheresis (ECP) may represent an alternative to immunosuppression, as a means of reducing rejection after thoracic organ transplantation. The mechanism by which ECP exerts its protective effects has, until now, remained elusive. We analyzed peripheral blood mononuclear cells of four children with chronic heart and lung transplant rejection, who received ECP in addition to conventional immunosuppressive treatment. The effects of ECP were evaluated at each cycle, comparing blood samples from the same patient collected before and after treatment. In vitro, peripheral blood mononuclear cells treated with ECP undergo apoptosis and are phagocytosed by immature dendritic cells, which, in turn, acquire a tolerogenic phenotype. The frequency of T cells, with a regulatory phenotype and strong suppressive activity, was significantly increased in the blood of ECP-treated patients. The immunomodulatory effects of ECP may be explained by its ability to increase the frequency of regulatory T cells with inhibitory action on transplant immune rejection.

Original languageEnglish
Pages (from-to)846-850
Number of pages5
JournalTransplantation
Volume79
Issue number7
DOIs
Publication statusPublished - Apr 15 2005

Fingerprint

Photopheresis
Regulatory T-Lymphocytes
Dendritic Cells
Graft Rejection
Blood Cells
Phenotype
Organ Transplantation
Immunosuppressive Agents
In Vitro Techniques
Phagocytosis
Immunosuppression
Thorax
Apoptosis
T-Lymphocytes
Lung
Therapeutics

Keywords

  • Apoptosis
  • Photopheresis
  • Regulatory T cells
  • Transplant rejection

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

The immunological effects of extracorporeal photopheresis unraveled : Induction of tolerogenic dendritic cells in vitro and regulatory T cells in vivo. / Lamioni, Andrea; Parisi, Francesco; Isacchi, Giancarlo; Giorda, Ezio; Di Cesare, Silvia; Landolfo, Attilio; Cenci, Francesco; Bottazzo, Gian Franco; Carsetti, Rita.

In: Transplantation, Vol. 79, No. 7, 15.04.2005, p. 846-850.

Research output: Contribution to journalArticle

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