The immunomodulatory protein SV-IV protects serum-deprived cells against apoptosis but not against G0/G1 arrest: Possible implications for the survival of implanting embryo

Francesco Morelli, Gianfranco Peluso, Orsolina Petillo, Angela Giannattasio, Silvana Filosa, Chiara M. Motta, Stefania Tammaro, Adriana Zatterale, Rita Calzone, Alfredo Budillon, Maria Cartenì, Salvatore De Maria, Maria R. Costanza, Antonella Nigro, Marcella Petrazzuolo, Elisabetta Buommino, Maria Rizzo, Giovanni Capasso, Salvatore Baiano, Francesco MoscatielloGianpietro Ravagnan, Maria P. Fuggetta, Gianfranco Tajana, Paola Stiuso, Bianca M. Metafora, Vittoria Metafora, Salvatore Metafora

Research output: Contribution to journalArticle

Abstract

Serum deprivation induced in human lymphoblastoid Raji cells oxidative stress-associated apoptotic death and G0/G1 cell cycle arrest. Addition into culture medium of the immunomodulatory protein Seminal vesicle protein 4 (SV-IV) protected these cells against apoptosis but not against cycle arrest. The antiapoptotic activity was related to: (1) decrease of endocellular reactive Oxygen species (ROS) (2) increase of mRNAs encoding anti-oxidant enzymes (catalase, G6PD) and antiapoptotic proteins (survivin, cox-1, Hsp70, c-Fos); (3) decrease of mRNAs encoding proapoptotic proteins (c-myc, Bax, caspase-3, Apaf-1). The biochemical changes underlaying these effects were probably induced by a protein tyrosine kinase (PTK) activity triggered by the binding of SV-IV to its putative plasma membrane receptors. The ineffectiveness of SV-IV to abrogate the cycle arrest was accounted for by its downregulating effects on D1,3/E G1-cyclins and CdK2/4 gene expression, ppRb/pRb ratio, and intracellular ROS concentration. In conclusion, these experiments: (1) prove that SV-IV acts as a cell survival factor; (2) suggest the involvement of a PTK in SV-IV signaling; (3) point to cell cycle-linked enzyme inhibition as responsible for cycle arrest; (4) provide a model to dissect the cycle arrest and apoptosis induced by serum withdrawal; (5) imply a possible role of SV-IV in the survival of hemiallogenic implanting embryos.

Original languageEnglish
Pages (from-to)610-625
Number of pages16
JournalJournal of Cellular Physiology
Volume212
Issue number3
DOIs
Publication statusPublished - Sep 2007

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Protein-Tyrosine Kinases
Seminal Vesicle Secretory Proteins
Reactive Oxygen Species
Embryonic Structures
Cyclin G1
Cells
Proto-Oncogene Proteins c-myc
Apoptosis
G1 Phase Cell Cycle Checkpoints
Cyclin E
Messenger RNA
Survival
Enzymes
Serum
Oxidants
Caspase 3
Catalase
Enzyme inhibition
Culture Media
Cell Survival

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

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The immunomodulatory protein SV-IV protects serum-deprived cells against apoptosis but not against G0/G1 arrest : Possible implications for the survival of implanting embryo. / Morelli, Francesco; Peluso, Gianfranco; Petillo, Orsolina; Giannattasio, Angela; Filosa, Silvana; Motta, Chiara M.; Tammaro, Stefania; Zatterale, Adriana; Calzone, Rita; Budillon, Alfredo; Cartenì, Maria; De Maria, Salvatore; Costanza, Maria R.; Nigro, Antonella; Petrazzuolo, Marcella; Buommino, Elisabetta; Rizzo, Maria; Capasso, Giovanni; Baiano, Salvatore; Moscatiello, Francesco; Ravagnan, Gianpietro; Fuggetta, Maria P.; Tajana, Gianfranco; Stiuso, Paola; Metafora, Bianca M.; Metafora, Vittoria; Metafora, Salvatore.

In: Journal of Cellular Physiology, Vol. 212, No. 3, 09.2007, p. 610-625.

Research output: Contribution to journalArticle

Morelli, F, Peluso, G, Petillo, O, Giannattasio, A, Filosa, S, Motta, CM, Tammaro, S, Zatterale, A, Calzone, R, Budillon, A, Cartenì, M, De Maria, S, Costanza, MR, Nigro, A, Petrazzuolo, M, Buommino, E, Rizzo, M, Capasso, G, Baiano, S, Moscatiello, F, Ravagnan, G, Fuggetta, MP, Tajana, G, Stiuso, P, Metafora, BM, Metafora, V & Metafora, S 2007, 'The immunomodulatory protein SV-IV protects serum-deprived cells against apoptosis but not against G0/G1 arrest: Possible implications for the survival of implanting embryo', Journal of Cellular Physiology, vol. 212, no. 3, pp. 610-625. https://doi.org/10.1002/jcp.21058
Morelli, Francesco ; Peluso, Gianfranco ; Petillo, Orsolina ; Giannattasio, Angela ; Filosa, Silvana ; Motta, Chiara M. ; Tammaro, Stefania ; Zatterale, Adriana ; Calzone, Rita ; Budillon, Alfredo ; Cartenì, Maria ; De Maria, Salvatore ; Costanza, Maria R. ; Nigro, Antonella ; Petrazzuolo, Marcella ; Buommino, Elisabetta ; Rizzo, Maria ; Capasso, Giovanni ; Baiano, Salvatore ; Moscatiello, Francesco ; Ravagnan, Gianpietro ; Fuggetta, Maria P. ; Tajana, Gianfranco ; Stiuso, Paola ; Metafora, Bianca M. ; Metafora, Vittoria ; Metafora, Salvatore. / The immunomodulatory protein SV-IV protects serum-deprived cells against apoptosis but not against G0/G1 arrest : Possible implications for the survival of implanting embryo. In: Journal of Cellular Physiology. 2007 ; Vol. 212, No. 3. pp. 610-625.
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abstract = "Serum deprivation induced in human lymphoblastoid Raji cells oxidative stress-associated apoptotic death and G0/G1 cell cycle arrest. Addition into culture medium of the immunomodulatory protein Seminal vesicle protein 4 (SV-IV) protected these cells against apoptosis but not against cycle arrest. The antiapoptotic activity was related to: (1) decrease of endocellular reactive Oxygen species (ROS) (2) increase of mRNAs encoding anti-oxidant enzymes (catalase, G6PD) and antiapoptotic proteins (survivin, cox-1, Hsp70, c-Fos); (3) decrease of mRNAs encoding proapoptotic proteins (c-myc, Bax, caspase-3, Apaf-1). The biochemical changes underlaying these effects were probably induced by a protein tyrosine kinase (PTK) activity triggered by the binding of SV-IV to its putative plasma membrane receptors. The ineffectiveness of SV-IV to abrogate the cycle arrest was accounted for by its downregulating effects on D1,3/E G1-cyclins and CdK2/4 gene expression, ppRb/pRb ratio, and intracellular ROS concentration. In conclusion, these experiments: (1) prove that SV-IV acts as a cell survival factor; (2) suggest the involvement of a PTK in SV-IV signaling; (3) point to cell cycle-linked enzyme inhibition as responsible for cycle arrest; (4) provide a model to dissect the cycle arrest and apoptosis induced by serum withdrawal; (5) imply a possible role of SV-IV in the survival of hemiallogenic implanting embryos.",
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AU - Petillo, Orsolina

AU - Giannattasio, Angela

AU - Filosa, Silvana

AU - Motta, Chiara M.

AU - Tammaro, Stefania

AU - Zatterale, Adriana

AU - Calzone, Rita

AU - Budillon, Alfredo

AU - Cartenì, Maria

AU - De Maria, Salvatore

AU - Costanza, Maria R.

AU - Nigro, Antonella

AU - Petrazzuolo, Marcella

AU - Buommino, Elisabetta

AU - Rizzo, Maria

AU - Capasso, Giovanni

AU - Baiano, Salvatore

AU - Moscatiello, Francesco

AU - Ravagnan, Gianpietro

AU - Fuggetta, Maria P.

AU - Tajana, Gianfranco

AU - Stiuso, Paola

AU - Metafora, Bianca M.

AU - Metafora, Vittoria

AU - Metafora, Salvatore

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