The immunoperoxidase technique has been applied to the rapid identification of human cytomegalovirus (CMV) strains on primary isolation. Negative and positive results have been constantly confirmed by indirect fluorescent antibody technique (IFA) and by electron microscopy. Using either the direct or indirect method it was observed that problems of nonspecific staining or uninfected cells were encountered mostly by the direct method, probably because of the lower working dilution of the conjugate (1:20) as compared with higher conjugate dilution used for the indirect method (1:150). However, nonspecific cytoplasmic stain of the Golgi area of infected cells was observed also by the indirect method. The pathogenesis of nonspecific labelling of this area is discussed. Application of the immunoperoxidase technique directly into tubes on primary isolation reduces identification time in comparison with the IFA technique and is less cumbersome and time consuming than complement fixation and neutralization tests.
|Translated title of the contribution||The immunoperoxidase technique for the rapid identification of human cytomegalovirus strains on primary isolation|
|Number of pages||17|
|Journal||Bollettino dell'Istituto Sieroterapico Milanese|
|Publication status||Published - 1975|
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