The immunosuppressant FK506 inhibits the damage to mouse pancreatic islets induced by low dose streptozocin

Gianpaolo Papaccio, Michael Latronico, Gabriella Chieffi Baccari

Research output: Contribution to journalArticlepeer-review

Abstract

Diabetes mellitus was induced in 40 male C57BL6 mice by injection of a low dose of streptozocin (45 mg/kg body weight) on 5 consecutive days. Twenty four of the mice were immunosuppressed by administration of 1.5 mg FK506/kg body weight daily for 10, 15, 18 and 24 days. Administration of FK506 almost completely inhibited the streptozocin-induced islet damage, and consequently glycaemia remained normal. In FK506-treated animals any inflammatory infiltrate was very sparse and was limited to the vascular pole of the islets. Immunocytochemical results demonstrated that infiltrating cells were Ia-immunoreactive, but were not activated. Ultrastructural observations confirmed the absence of B cell necrosis and degranulation in FK506-treated mice; the few infiltrating elements encountered did not contain phagocytic vesicles or show other signs of activation.

Original languageEnglish
Pages (from-to)573-578
Number of pages6
JournalCell & Tissue Research
Volume277
Issue number3
DOIs
Publication statusPublished - Sep 1994

Keywords

  • B cell
  • FK506
  • Immunosuppression
  • Mouse C57BL6
  • Pancreatic islet
  • Streptozocin
  • Type 1 diabetes

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

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