The importance of 'like to like' ISI calibrations with freeze dried plasmas

L. Poller, A. M H P Van Den Besselaar, J. Jespersen, A. Tripodi, D. Houghton

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Aim - To assess reliability of like to like and cross species calibrations using two types of certified freeze dried plasma calibrants- artificially depleted of vitamin K clotting factors, and from coumarin treated patients. Methods - Six ECAA national control laboratories provided certified values for the freeze dried plasmas in terms of the human plain international reference preparation (IRP) (BCT/441) with the manual prothrombin time technique. Eight other ECAA national laboratories determined international sensitivity index (ISI) values in full fresh plasma same species and cross species WHO calibrations against a low ISI human IRP (BCT/441) of the ECAA low ISI human thromboplastin and high ISI ECAA rabbit thromboplastin. Parallel calibrations were performed using the certified values. Results - Calibrations on fresh plasmas of the human ECAA reference thromboplastin (stated ISI = 0.95) gave ISI of 0.957 against the human IRP and 1.66 against the rabbit IRP. The ECAA rabbit (stated ISI = 1.67) gave an identical value on the fresh plasma calibration v the human IRP. With freeze dried depleted plasmas certified in terms of the human IRP, the ISI of the ECAA human was 1.01, but the ECAA rabbit (stated ISI = 1.67) gave a low ISI of 1.47. The freeze dried coumarin plasmas gave an ISI of 0.943 for the ECAA human but only 1.493 for the ECAA rabbit. Conclusions - Fresh plasmas give reliable ISI when calibrating thromboplastins in same species and cross species calibrations. Freeze dried plasmas certified in terms of a single IRP, whether artificially depleted or of coumarin plasma origin, cannot be used for calibration of dissimilar thromboplastins.

Original languageEnglish
Pages (from-to)275-279
Number of pages5
JournalJournal of Clinical Pathology
Volume51
Issue number4
Publication statusPublished - 1998

Fingerprint

Calibration
Thromboplastin
Rabbits
Blood Coagulation Factors
Vitamin K
Prothrombin Time

Keywords

  • Freeze dried plasma
  • International sensitivity index
  • Species specificity
  • Thromboplastin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Poller, L., Van Den Besselaar, A. M. H. P., Jespersen, J., Tripodi, A., & Houghton, D. (1998). The importance of 'like to like' ISI calibrations with freeze dried plasmas. Journal of Clinical Pathology, 51(4), 275-279.

The importance of 'like to like' ISI calibrations with freeze dried plasmas. / Poller, L.; Van Den Besselaar, A. M H P; Jespersen, J.; Tripodi, A.; Houghton, D.

In: Journal of Clinical Pathology, Vol. 51, No. 4, 1998, p. 275-279.

Research output: Contribution to journalArticle

Poller, L, Van Den Besselaar, AMHP, Jespersen, J, Tripodi, A & Houghton, D 1998, 'The importance of 'like to like' ISI calibrations with freeze dried plasmas', Journal of Clinical Pathology, vol. 51, no. 4, pp. 275-279.
Poller L, Van Den Besselaar AMHP, Jespersen J, Tripodi A, Houghton D. The importance of 'like to like' ISI calibrations with freeze dried plasmas. Journal of Clinical Pathology. 1998;51(4):275-279.
Poller, L. ; Van Den Besselaar, A. M H P ; Jespersen, J. ; Tripodi, A. ; Houghton, D. / The importance of 'like to like' ISI calibrations with freeze dried plasmas. In: Journal of Clinical Pathology. 1998 ; Vol. 51, No. 4. pp. 275-279.
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AU - Houghton, D.

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N2 - Aim - To assess reliability of like to like and cross species calibrations using two types of certified freeze dried plasma calibrants- artificially depleted of vitamin K clotting factors, and from coumarin treated patients. Methods - Six ECAA national control laboratories provided certified values for the freeze dried plasmas in terms of the human plain international reference preparation (IRP) (BCT/441) with the manual prothrombin time technique. Eight other ECAA national laboratories determined international sensitivity index (ISI) values in full fresh plasma same species and cross species WHO calibrations against a low ISI human IRP (BCT/441) of the ECAA low ISI human thromboplastin and high ISI ECAA rabbit thromboplastin. Parallel calibrations were performed using the certified values. Results - Calibrations on fresh plasmas of the human ECAA reference thromboplastin (stated ISI = 0.95) gave ISI of 0.957 against the human IRP and 1.66 against the rabbit IRP. The ECAA rabbit (stated ISI = 1.67) gave an identical value on the fresh plasma calibration v the human IRP. With freeze dried depleted plasmas certified in terms of the human IRP, the ISI of the ECAA human was 1.01, but the ECAA rabbit (stated ISI = 1.67) gave a low ISI of 1.47. The freeze dried coumarin plasmas gave an ISI of 0.943 for the ECAA human but only 1.493 for the ECAA rabbit. Conclusions - Fresh plasmas give reliable ISI when calibrating thromboplastins in same species and cross species calibrations. Freeze dried plasmas certified in terms of a single IRP, whether artificially depleted or of coumarin plasma origin, cannot be used for calibration of dissimilar thromboplastins.

AB - Aim - To assess reliability of like to like and cross species calibrations using two types of certified freeze dried plasma calibrants- artificially depleted of vitamin K clotting factors, and from coumarin treated patients. Methods - Six ECAA national control laboratories provided certified values for the freeze dried plasmas in terms of the human plain international reference preparation (IRP) (BCT/441) with the manual prothrombin time technique. Eight other ECAA national laboratories determined international sensitivity index (ISI) values in full fresh plasma same species and cross species WHO calibrations against a low ISI human IRP (BCT/441) of the ECAA low ISI human thromboplastin and high ISI ECAA rabbit thromboplastin. Parallel calibrations were performed using the certified values. Results - Calibrations on fresh plasmas of the human ECAA reference thromboplastin (stated ISI = 0.95) gave ISI of 0.957 against the human IRP and 1.66 against the rabbit IRP. The ECAA rabbit (stated ISI = 1.67) gave an identical value on the fresh plasma calibration v the human IRP. With freeze dried depleted plasmas certified in terms of the human IRP, the ISI of the ECAA human was 1.01, but the ECAA rabbit (stated ISI = 1.67) gave a low ISI of 1.47. The freeze dried coumarin plasmas gave an ISI of 0.943 for the ECAA human but only 1.493 for the ECAA rabbit. Conclusions - Fresh plasmas give reliable ISI when calibrating thromboplastins in same species and cross species calibrations. Freeze dried plasmas certified in terms of a single IRP, whether artificially depleted or of coumarin plasma origin, cannot be used for calibration of dissimilar thromboplastins.

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