The increased expression of HLA-DR and ICAM-1 molecules by human bronchial epithelial cells, induced by activated mononuclear cells, is downregulated by nedocromil sodium

O. Sacco, S. Lantero, V. Frangova, V. Ottolini, G. A. Rossi, L. Scarso

Research output: Contribution to journalArticle

Abstract

To test the hypothesis that mononuclear cell products could increase the expression of HLA-DR and ICAM-1 molecules in bronchial epithelial cells (BECs), subconfluent cultures of human BECs, obtained from surgically resected bronchi, were incubated with PHA-activated blood mononuclear cell conditioned media (BCM-CM) or recombinant EFN-γ. The presence of HLA-DR and ICAM-1 molecules on BECs was then evaluated by specific antibody staining and flow-cytometry analysis. The addition to BEC cultures of different concentrations of PHA-stimulated BMC-CM, or of EFN-γ, induced a dose-dependent increase of HLA-DR and ICAM-1 expression, while no effect was observed with unstimulated BMC-CM. The ability of nedocromil sodium and, as control, of dexamethasone, to prevent the upregulation of HLA-DR and ICAM-1 expression on BECs was then tested. Increasing concentrations (10–7 to 10–4 M) of nedocromil significantly inhibited HLA-DR and ICAM-1 expression by BECs in a dose-dependent fashion. A similarly dose-dependent inhibitory effect was also observed with dexamethasone, which, however, was less active than nedocromil on HLA-DR expression and more active on ICAM-1 expression. Finally, nedocromil and dexamethasone showed a significant synergistic effect on the expression of both cell surface molecules at the lowest concentrations tested.

Original languageEnglish
Pages (from-to)S7-S13
JournalMediators of Inflammation
Volume3
Issue number7
DOIs
Publication statusPublished - 1994

Keywords

  • Adhesion molecules
  • Bronchial epithelial cells
  • Cromones
  • HLA-DR
  • ICAM-1
  • Interferon-γ

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

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