The induction of apoptosis is a common feature of the cytotoxic action of ether-linked glycerophospholipids in human leukemic cells

L. Diomede, B. Piovani, F. Re, P. Principe, F. Colotta, E. J. Modest, M. Salmona

Research output: Contribution to journalArticlepeer-review

Abstract

The ability of 2 recent ether-lipid derivatives, aza-phospholipids BN52205 and BN52211, to induce apoptosis in different leukemia cell lines was investigated using 1-octadecyl-2-methyl-rac-glycero-3-phosphocholine (ET-18- OCH3) as a positive control. HL60, K562, Molt-4 and U937 cells were exposed for 24 hr to 20 μM of drug. The 2 aza-derivatives were as cytotoxic as ET- 18-OCH3: BN52205 and BN52211 selectively induced apoptotic death in HL60, Molt-4 and U937 cells, but not in the K562-resistant cell line. Around 50% of DNA was fragmented in HL60 cells after exposure to the aza-derivatives, and 34% and 20% of DNA was fragmented in Molt-4 and U937 cells respectively. Similar results were obtained when cells were exposed to ET-18-OCH3. Our data confirm that ether lipids induce apoptosis in a variety of human leukemic cells, providing a possible explanation for their selectivity and mechanism of action.

Original languageEnglish
Pages (from-to)645-649
Number of pages5
JournalInternational Journal of Cancer
Volume57
Issue number5
DOIs
Publication statusPublished - 1994

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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