The influence of surgery, immunosuppressive drugs, and rejection, on graft function after small bowel transplantation: A large-animal study

Mario Alessiani, Fabrizio De Ponti, Filiberto Fayer, Francesca Abbiati, Sandro Zonta, Ermanno Zitelli, Eloisa Arbustini, Patrizia Morbini, Nicoletta Poggi, Catherine Klersy, Ilaria Blangetti, Paolo Dionigi, Aris Zonta

Research output: Contribution to journalArticle

Abstract

In this study we assessed functional changes (motility and absorption) of intestinal allografts in a large-animal model of orthotopic small bowel transplantation in swine. Studies were performed on non-rejecting animals in the early and late stages after transplantation and after induction of different grades of acute rejection. Immunosuppression consisted of oral FK506 and mycophenolate mofetil. In each study group we regulated drug administration, in terms of dosage and timing, in order to induce different grades of acute rejection or to prevent it. Migrating myoelectrical complexes were recorded in fasting animals so that motility could be assessed. Mucosal biopsy of the allograft and D-xylose absorption tests were performed on the same day as the motility study. In the early stages following intestinal transplantation, we observed in non-rejecting animals a slightly increased graft motility and a marked carbohydrate malabsorption. Recovery of the carbohydrate absorption capacity occurs within 2 months, but the persistence of diarrhea leads to partial malabsorption and to a lack of normal weight gain. Motility reduction correlates with the grade of acute rejection and becomes significant at a later stage, when rejection is severe. Allograft carbohydrate absorption, on the contrary, is markedly reduced in all rejecting pigs, irrespective of the grade of rejection. In summary, the early functional impairment of non-rejecting animals has multifactorial causes due to surgery and immunosuppression (drug toxicity), and its occurrence suggests the need for specific guidelines for clinical early postoperative enteral feeding. The functional studies adopted here are helpful in defining the grade of functional impairment with or without acute rejection; however, they are not useful for early detection of ongoing acute rejection of the small bowel graft.

Original languageEnglish
Pages (from-to)327-335
Number of pages9
JournalTransplant International
Volume16
Issue number5
DOIs
Publication statusPublished - May 1 2003

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Graft Rejection
Immunosuppressive Agents
Transplantation
Pharmaceutical Preparations
Allografts
Carbohydrates
Immunosuppression
Swine
Mycophenolic Acid
Transplants
Gastrointestinal Motility
Xylose
Intestinal Absorption
Enteral Nutrition
Tacrolimus
Rejection (Psychology)
Drug-Related Side Effects and Adverse Reactions
Weight Gain
Diarrhea
Fasting

Keywords

  • Absorption
  • Acute cellular rejection
  • Experimental model
  • Intestinal transplantation
  • Motility

ASJC Scopus subject areas

  • Transplantation

Cite this

The influence of surgery, immunosuppressive drugs, and rejection, on graft function after small bowel transplantation : A large-animal study. / Alessiani, Mario; De Ponti, Fabrizio; Fayer, Filiberto; Abbiati, Francesca; Zonta, Sandro; Zitelli, Ermanno; Arbustini, Eloisa; Morbini, Patrizia; Poggi, Nicoletta; Klersy, Catherine; Blangetti, Ilaria; Dionigi, Paolo; Zonta, Aris.

In: Transplant International, Vol. 16, No. 5, 01.05.2003, p. 327-335.

Research output: Contribution to journalArticle

Alessiani, Mario ; De Ponti, Fabrizio ; Fayer, Filiberto ; Abbiati, Francesca ; Zonta, Sandro ; Zitelli, Ermanno ; Arbustini, Eloisa ; Morbini, Patrizia ; Poggi, Nicoletta ; Klersy, Catherine ; Blangetti, Ilaria ; Dionigi, Paolo ; Zonta, Aris. / The influence of surgery, immunosuppressive drugs, and rejection, on graft function after small bowel transplantation : A large-animal study. In: Transplant International. 2003 ; Vol. 16, No. 5. pp. 327-335.
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