The inhibitor of IkappaBalpha phosphorylation BAY 11-7082 prevents NMDA neurotoxicity in mouse hippocampal slices

F. Goffi, F. Boroni, M. Benarese, I. Sarnico, A. Benetti, P. F. Spano, M. Pizzi

Research output: Contribution to journalArticle

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Abstract

NF-κB is a nuclear transcription factor involved in the control of fundamental cellular functions including cell survival. Among the many target genes of this factor, both pro- and anti-apoptotic genes have been described. To evaluate the contribution of NF-κB activation to excitotoxic insult, we analysed the effect of IkappaBalpha (IκBα) phosphorylation blockade on glutamate-induced toxicity in adult mouse hippocampal slices. By using immunocytochemical and EMSA techniques, we found that (i) acute exposure of hippocampal slices to NMDA induced nuclear translocation of NF-κB, (ii) NMDA-mediated activation of NF-κB was prevented by BAY 11-7082, an inhibitor of IκBα phosphorylation and degradation, and (iii) BAY 11-7082-mediated inhibition of NF-κB activation was associated with neuroprotection.

Original languageEnglish
Pages (from-to)147-151
Number of pages5
JournalNeuroscience Letters
Volume377
Issue number3
DOIs
Publication statusPublished - Apr 4 2005

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N-Methylaspartate
Phosphorylation
Genes
Glutamic Acid
Cell Survival
Transcription Factors
3-(4-methylphenylsulfonyl)-2-propenenitrile
NF-KappaB Inhibitor alpha
Neuroprotection

Keywords

  • BAY 11-7082
  • Neuroprotection
  • NF-κB
  • NMDA

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Goffi, F., Boroni, F., Benarese, M., Sarnico, I., Benetti, A., Spano, P. F., & Pizzi, M. (2005). The inhibitor of IkappaBalpha phosphorylation BAY 11-7082 prevents NMDA neurotoxicity in mouse hippocampal slices. Neuroscience Letters, 377(3), 147-151. https://doi.org/10.1016/j.neulet.2004.11.088

The inhibitor of IkappaBalpha phosphorylation BAY 11-7082 prevents NMDA neurotoxicity in mouse hippocampal slices. / Goffi, F.; Boroni, F.; Benarese, M.; Sarnico, I.; Benetti, A.; Spano, P. F.; Pizzi, M.

In: Neuroscience Letters, Vol. 377, No. 3, 04.04.2005, p. 147-151.

Research output: Contribution to journalArticle

Goffi, F, Boroni, F, Benarese, M, Sarnico, I, Benetti, A, Spano, PF & Pizzi, M 2005, 'The inhibitor of IkappaBalpha phosphorylation BAY 11-7082 prevents NMDA neurotoxicity in mouse hippocampal slices', Neuroscience Letters, vol. 377, no. 3, pp. 147-151. https://doi.org/10.1016/j.neulet.2004.11.088
Goffi, F. ; Boroni, F. ; Benarese, M. ; Sarnico, I. ; Benetti, A. ; Spano, P. F. ; Pizzi, M. / The inhibitor of IkappaBalpha phosphorylation BAY 11-7082 prevents NMDA neurotoxicity in mouse hippocampal slices. In: Neuroscience Letters. 2005 ; Vol. 377, No. 3. pp. 147-151.
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