The inhibitor of IkappaBalpha phosphorylation BAY 11-7082 prevents NMDA neurotoxicity in mouse hippocampal slices

F. Goffi, F. Boroni, M. Benarese, I. Sarnico, A. Benetti, P. F. Spano, M. Pizzi

Research output: Contribution to journalArticle

Abstract

NF-κB is a nuclear transcription factor involved in the control of fundamental cellular functions including cell survival. Among the many target genes of this factor, both pro- and anti-apoptotic genes have been described. To evaluate the contribution of NF-κB activation to excitotoxic insult, we analysed the effect of IkappaBalpha (IκBα) phosphorylation blockade on glutamate-induced toxicity in adult mouse hippocampal slices. By using immunocytochemical and EMSA techniques, we found that (i) acute exposure of hippocampal slices to NMDA induced nuclear translocation of NF-κB, (ii) NMDA-mediated activation of NF-κB was prevented by BAY 11-7082, an inhibitor of IκBα phosphorylation and degradation, and (iii) BAY 11-7082-mediated inhibition of NF-κB activation was associated with neuroprotection.

Original languageEnglish
Pages (from-to)147-151
Number of pages5
JournalNeuroscience Letters
Volume377
Issue number3
DOIs
Publication statusPublished - Apr 4 2005

Keywords

  • BAY 11-7082
  • Neuroprotection
  • NF-κB
  • NMDA

ASJC Scopus subject areas

  • Neuroscience(all)

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    Goffi, F., Boroni, F., Benarese, M., Sarnico, I., Benetti, A., Spano, P. F., & Pizzi, M. (2005). The inhibitor of IkappaBalpha phosphorylation BAY 11-7082 prevents NMDA neurotoxicity in mouse hippocampal slices. Neuroscience Letters, 377(3), 147-151. https://doi.org/10.1016/j.neulet.2004.11.088