The inhibitory effect of serum from hairy-cell leukaemia patients on normal progenitor cells may disappear following prolonged treatment with α-interferon

F. Lauria, G. P. Bagnara, L. Catani, L. Gaggioli, A. Guarini, D. Raspadori, R. Foa, G. Bellone, M. Buzzi, L. Gugliotta, G. Zauli, M. Giovanni, P. L. Zinzani, S. Tura

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Abstract

The possibility that serum from hairy-cell leukaemia (HCL) patients at diagnosis may show an inhibitory effect on the in vitro colony growth of normal haemopoietic progenitor cells has been suggested. Several studies have documented the efficacy of α-Interferon (α-IFN) in inducing a complete restoration of peripheral blood values and, in some cases, a complete clinical remission. In this study we have evaluated the regulatory effect of serum, collected before and after 3 and 12 months of α-IFN treatment, from 10 patients with untreated HCL, on the in vitro growth of normal bone marrow CFU-GM, BFU-E, and CFU-MK. The effect of conditioned media, prepared from enriched hairy cells (HC) cultured in synthetic medium, on the growth of normal haemopoietic progenitors was also investigated. The results obtained confirm that sera from untreated HCL patients display a variable degree of inhibitory activity in the progenitor cell compartments analysed. Disappearance of the inhibitory activity, particularly evident for the erythroid compartment, was found only in patients who displayed a diappearance of circulating HC and a good haematological response after prolonged (12 months) treatment with α-IFN. The possibility that the serum of patients with HCL may contain a haemopoietic inhibitory factor, released by the neoplastic HC population, is suggested.

Original languageEnglish
Pages (from-to)497-501
Number of pages5
JournalBritish Journal of Haematology
Volume72
Issue number4
Publication statusPublished - 1989

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Hairy Cell Leukemia
Interferons
Stem Cells
Serum
Growth
Therapeutics
Granulocyte-Macrophage Progenitor Cells
Erythroid Precursor Cells
Conditioned Culture Medium
Cultured Cells
Bone Marrow
Population

ASJC Scopus subject areas

  • Hematology

Cite this

The inhibitory effect of serum from hairy-cell leukaemia patients on normal progenitor cells may disappear following prolonged treatment with α-interferon. / Lauria, F.; Bagnara, G. P.; Catani, L.; Gaggioli, L.; Guarini, A.; Raspadori, D.; Foa, R.; Bellone, G.; Buzzi, M.; Gugliotta, L.; Zauli, G.; Giovanni, M.; Zinzani, P. L.; Tura, S.

In: British Journal of Haematology, Vol. 72, No. 4, 1989, p. 497-501.

Research output: Contribution to journalArticle

Lauria, F, Bagnara, GP, Catani, L, Gaggioli, L, Guarini, A, Raspadori, D, Foa, R, Bellone, G, Buzzi, M, Gugliotta, L, Zauli, G, Giovanni, M, Zinzani, PL & Tura, S 1989, 'The inhibitory effect of serum from hairy-cell leukaemia patients on normal progenitor cells may disappear following prolonged treatment with α-interferon', British Journal of Haematology, vol. 72, no. 4, pp. 497-501.
Lauria, F. ; Bagnara, G. P. ; Catani, L. ; Gaggioli, L. ; Guarini, A. ; Raspadori, D. ; Foa, R. ; Bellone, G. ; Buzzi, M. ; Gugliotta, L. ; Zauli, G. ; Giovanni, M. ; Zinzani, P. L. ; Tura, S. / The inhibitory effect of serum from hairy-cell leukaemia patients on normal progenitor cells may disappear following prolonged treatment with α-interferon. In: British Journal of Haematology. 1989 ; Vol. 72, No. 4. pp. 497-501.
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AU - Foa, R.

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AU - Zinzani, P. L.

AU - Tura, S.

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