The inner side of T cell lipid rafts

Giorgia Gri, Barbara Molon, Santos Manes, Tullio Pozzan, Antonella Viola

Research output: Contribution to journalArticlepeer-review

Abstract

A key question in understanding the functional role of lipid rafts is whether lipid microdomains at the plasma membrane outer leaflet are coupled to lipid microdomains at the inner leaflet. By using a cyan-fluorescent protein (CFP) targeted to inner plasma membrane rafts of Jurkat T cells, we found that raft domains at the outer and inner leaflets are physically coupled and that this coupling requires cholesterol. Interestingly, TCR/CD3 cross-linking induces co-capping of the raft bilayer independently of cholesterol or signaling events, indicating that cholesterol-extracting drugs are unable to destroy TCR-lipid rafts interaction.

Original languageEnglish
Pages (from-to)247-252
Number of pages6
JournalImmunology Letters
Volume94
Issue number3
DOIs
Publication statusPublished - Jul 15 2004

Keywords

  • CFP
  • cholera toxin B subunit
  • CT-B
  • cyan-fluorescent protein
  • glycosylphosphatidylinositol
  • GPI
  • Lipid rafts
  • MβCD
  • methyl-β- cyclodextrin
  • T cell receptor
  • T lymphocyte
  • TCR

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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