The inositol 1,4,5-trisphosphate-generating agonist ATP enhances DNA cleavage induced by tert-butylhydroperoxide

Emilio Clementi, Andrea Guidarelli, Orazio Cantoni

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In this paper we present experimental evidence indicating that DNA cleavage induced by tert-butylhydroperoxide in U937 cells can be enhanced via ATP-mediated activation of membrane receptors coupled with hydrolysis of phosphatidylinositol 4,5-bisphosphate. The mechanism whereby ATP exerts this effect involves release of Ca2+ from the inositol 1,4,5-trisphosphate (IP3)-sensitive stores, further release of the cation from the ryanodine receptor, mitochondrial clearance of the fraction of Ca2+ derived from the ryanodine receptor, and Ca2+-dependent mitochondrial formation of DNA- damaging species. IP3-generating agonists must therefore be considered as potential modulators of the genotoxic effects of tert-butylhy-droperoxide.

Original languageEnglish
Pages (from-to)175-178
Number of pages4
JournalExperimental Cell Research
Volume239
Issue number1
DOIs
Publication statusPublished - Feb 25 1998

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tert-Butylhydroperoxide
DNA Cleavage
Ryanodine Receptor Calcium Release Channel
Inositol 1,4,5-Trisphosphate
Adenosine Triphosphate
U937 Cells
Phosphatidylinositols
Mitochondrial DNA
Cations
Hydrolysis
Membranes

ASJC Scopus subject areas

  • Cell Biology

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The inositol 1,4,5-trisphosphate-generating agonist ATP enhances DNA cleavage induced by tert-butylhydroperoxide. / Clementi, Emilio; Guidarelli, Andrea; Cantoni, Orazio.

In: Experimental Cell Research, Vol. 239, No. 1, 25.02.1998, p. 175-178.

Research output: Contribution to journalArticle

Clementi, Emilio ; Guidarelli, Andrea ; Cantoni, Orazio. / The inositol 1,4,5-trisphosphate-generating agonist ATP enhances DNA cleavage induced by tert-butylhydroperoxide. In: Experimental Cell Research. 1998 ; Vol. 239, No. 1. pp. 175-178.
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