The interacting RNA polymerase II subunits, hRPB11 and hRPB3, are coordinately expressed in adult human tissues and down-regulated by doxorubicin

Maurizio Fanciulli, Tiziana Bruno, Monica Di Padova, Roberta De Angelis, Sarah Lovari, Aristide Floridi, Claudio Passananti

Research output: Contribution to journalArticlepeer-review

Abstract

We previously isolated the human RPB11 cDNA, encoding the 13.3 kDa subunit of RNA polymerase II, and demonstrated that expression of this subunit is modulated by doxorubicin. Using hRPB11 as bait in a yeast two-hybrid system, two cDNA variants encoding a second RNA polymerase II subunit, hRP93, have now been isolated and characterized. These two hRPB3 mRNA species differed in 3' UTR region length, the longer transcript containing the AU-rich sequence motif that mediates mRNA degradation. Both hRPB11 and hRPB3 transcripts share a similar pattern of distribution in human adult tissues, with particularly high levels in both heart and skeletal muscle, and the expression of both is down-regulated by doxorubicin as found previously for the hRPB11 subunit. Taken together, these findings suggest that the interaction between hRPB3 and hRPB11 is fundamental for their function and that this heterodimer is involved in doxorubicin toxicity.

Original languageEnglish
Pages (from-to)236-240
Number of pages5
JournalFEBS Letters
Volume427
Issue number2
DOIs
Publication statusPublished - May 8 1998

Keywords

  • Alternative splicing
  • Doxorubicin
  • Drug toxicity
  • MRNA degradation
  • RNA polymerase II
  • Two-hybrid system

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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