The interference of rosmarinic acid in the DNA fragmentation induced by osmotic shock

Paola Sinibaldi Salimei, Gabriella Marfè, Livia Di Renzo, Carla Di Stefano, Maria Gabriella Giganti, Giuseppe Filomeni, Maria Rosa Ciriolo

Research output: Contribution to journalArticlepeer-review

Abstract

The induction of cell death in human erythroleukemic cells (K562) by sorbitol shows the typical apoptotic changes in ultrastructural morphology, including blebbing, chromatin condensation and nuclear membrane breakdown.. Using a cytofluorimetric approach, we found that sorbitol induced production of reactive oxygen species (ROS) followed by DNA fragmentation in leukemic cells. In this study, we investigated effect of curcumin and rosmarinic acid on cell viability in three different cell lines: erythroleukemia K562, papillary NPA, and anaplastic ARO thyroid cancers. Curcumin was able to induce apoptosis in a concentration- and time dependent manner in three cell lines, while rosmarinic acid was less effective on this process. To examine this possibility in cellular system, this study evaluated the capacities of both compounds acting as antioxidant inhibiting sorbitolinduced apoptosis. K562, NPA and ARO cells were preincubated with 25 μM rosmarinic acid to allow the uptake and then the cell lines were treated with l M sorbitol. Afterwards, the cells were subjected to agarose gel electrophoresis to assess the DNA fragmentation. In conclusion, the antioxidant activity of rosmarinic acid is able to inhibit sorbitol-induced apoptosis. curcumin, rosmarinic acid, sorbitol, apoptosis, ROS.

Original languageEnglish
Pages (from-to)1308-1317
Number of pages10
JournalFrontiers in Bioscience
Volume12
Issue number4
DOIs
Publication statusPublished - 2007

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

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