TY - JOUR
T1 - The interference of rosmarinic acid in the DNA fragmentation induced by osmotic shock
AU - Salimei, Paola Sinibaldi
AU - Marfè, Gabriella
AU - Di Renzo, Livia
AU - Di Stefano, Carla
AU - Giganti, Maria Gabriella
AU - Filomeni, Giuseppe
AU - Ciriolo, Maria Rosa
PY - 2007
Y1 - 2007
N2 - The induction of cell death in human erythroleukemic cells (K562) by sorbitol shows the typical apoptotic changes in ultrastructural morphology, including blebbing, chromatin condensation and nuclear membrane breakdown.. Using a cytofluorimetric approach, we found that sorbitol induced production of reactive oxygen species (ROS) followed by DNA fragmentation in leukemic cells. In this study, we investigated effect of curcumin and rosmarinic acid on cell viability in three different cell lines: erythroleukemia K562, papillary NPA, and anaplastic ARO thyroid cancers. Curcumin was able to induce apoptosis in a concentration- and time dependent manner in three cell lines, while rosmarinic acid was less effective on this process. To examine this possibility in cellular system, this study evaluated the capacities of both compounds acting as antioxidant inhibiting sorbitolinduced apoptosis. K562, NPA and ARO cells were preincubated with 25 μM rosmarinic acid to allow the uptake and then the cell lines were treated with l M sorbitol. Afterwards, the cells were subjected to agarose gel electrophoresis to assess the DNA fragmentation. In conclusion, the antioxidant activity of rosmarinic acid is able to inhibit sorbitol-induced apoptosis. curcumin, rosmarinic acid, sorbitol, apoptosis, ROS.
AB - The induction of cell death in human erythroleukemic cells (K562) by sorbitol shows the typical apoptotic changes in ultrastructural morphology, including blebbing, chromatin condensation and nuclear membrane breakdown.. Using a cytofluorimetric approach, we found that sorbitol induced production of reactive oxygen species (ROS) followed by DNA fragmentation in leukemic cells. In this study, we investigated effect of curcumin and rosmarinic acid on cell viability in three different cell lines: erythroleukemia K562, papillary NPA, and anaplastic ARO thyroid cancers. Curcumin was able to induce apoptosis in a concentration- and time dependent manner in three cell lines, while rosmarinic acid was less effective on this process. To examine this possibility in cellular system, this study evaluated the capacities of both compounds acting as antioxidant inhibiting sorbitolinduced apoptosis. K562, NPA and ARO cells were preincubated with 25 μM rosmarinic acid to allow the uptake and then the cell lines were treated with l M sorbitol. Afterwards, the cells were subjected to agarose gel electrophoresis to assess the DNA fragmentation. In conclusion, the antioxidant activity of rosmarinic acid is able to inhibit sorbitol-induced apoptosis. curcumin, rosmarinic acid, sorbitol, apoptosis, ROS.
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U2 - 10.2741/2149
DO - 10.2741/2149
M3 - Article
C2 - 17127383
AN - SCOPUS:33947509806
VL - 12
SP - 1308
EP - 1317
JO - Frontiers in Bioscience - Landmark
JF - Frontiers in Bioscience - Landmark
SN - 1093-9946
IS - 4
ER -