The intron-containing L3 ribosomal protein gene (RPL3): Sequence analysis and identification of U43 and of two novel intronic small nucleolar RNAs

Stefano Duga, Rosanna Asselta, Massimo Malcovati, Maria L. Tenchini, Severino Ronchi, Tatjana Simonic

Research output: Contribution to journalArticlepeer-review

Abstract

Isolation and sequencing of bovine and human intron-containing L3 ribosomal protein genes are here reported. They exhibit very similar organisation, both comprising 10 exons and nine introns. A polymorphic locus, involving a 19-bp deletion, was found in intron 6 of the human gene. The frequency of the two alleles has been estimated in 200 haploid genomes. In bovine and human genes intron sequences are rather different, except for limited regions, located in corresponding positions, which show a surprisingly high degree of identity. All these regions contain conserved features defining the box C/D class of small nucleolar RNAs. Demonstration is given that U43 small nucleolar RNA is encoded within the first intron of both bovine and human genes. Single nucleotide sequences, encoding two novel species of small nucleolar RNAs (U82, U83a and U83b), are located in introns 3, 5 and 7. Their expression has been investigated and a possible role of these molecules in 2'-O-ribose methylation of rRNAs is discussed. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)225-236
Number of pages12
JournalBBA - Gene Structure and Expression
Volume1490
Issue number3
DOIs
Publication statusPublished - Feb 29 2000

Keywords

  • Gene structure
  • L3 ribosomal protein
  • Small nucleolar RNA
  • U43
  • U82
  • U83

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

Fingerprint Dive into the research topics of 'The intron-containing L3 ribosomal protein gene (RPL3): Sequence analysis and identification of U43 and of two novel intronic small nucleolar RNAs'. Together they form a unique fingerprint.

Cite this