The isolated and perfused brain of the guinea-pig in vitro

M. Muhlethaler, M. de Curtis, K. Walton, R. Llinas

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

We describe here an isolated and perfused in vitro adult guinea-pig whole brain preparation which is an extension of the previously described in vitro brainstem - cerebellum preparation. Viability was tested by the analysis of trans-synaptic responses along the visual pathways following the electrical stimulation of the optic nerve or the optic radiations. The evoked field potentials were recorded in the dorsal lateral geniculate, the superior colliculus and the visual cortex. The distribution of extracellular currents was studied using current source density analysis, in order to determine the amplitude, time course and spatial organization of the synaptic activity at these sites. The study indicates that field potentials were very similar to those described in vivo. These data demonstrate the survival of a complex adult sensory system in vitro and suggest that this preparation can be used for the analysis of multisynaptic circuits in the mammalian brain.

Original languageEnglish
Pages (from-to)915-926
Number of pages12
JournalEuropean Journal of Neuroscience
Volume5
Issue number7
Publication statusPublished - 1993

Fingerprint

Guinea Pigs
Brain
Visual Pathways
Superior Colliculi
Visual Cortex
Optic Nerve
Evoked Potentials
Cerebellum
Electric Stimulation
Brain Stem
Radiation
In Vitro Techniques

Keywords

  • Superior colliculus
  • Visual system

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The isolated and perfused brain of the guinea-pig in vitro. / Muhlethaler, M.; de Curtis, M.; Walton, K.; Llinas, R.

In: European Journal of Neuroscience, Vol. 5, No. 7, 1993, p. 915-926.

Research output: Contribution to journalArticle

Muhlethaler, M, de Curtis, M, Walton, K & Llinas, R 1993, 'The isolated and perfused brain of the guinea-pig in vitro', European Journal of Neuroscience, vol. 5, no. 7, pp. 915-926.
Muhlethaler, M. ; de Curtis, M. ; Walton, K. ; Llinas, R. / The isolated and perfused brain of the guinea-pig in vitro. In: European Journal of Neuroscience. 1993 ; Vol. 5, No. 7. pp. 915-926.
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