The isolated carboxy-terminal domain of human mitochondrial leucyl-tRNA synthetase rescues the pathological phenotype of mitochondrial tRNA mutations in human cells

Elena Perli, Carla Giordano, Annalinda Pisano, Arianna Montanari, Antonio F. Campese, Aurelio Reyes, Daniele Ghezzi, Alessia Nasca, Helen A. Tuppen, Maurizia Orlandi, Patrizio Di Micco, Elena Poser, Robert W. Taylor, Gianni Colotti, Silvia Francisci, Veronica Morea, Laura Frontali, Massimo Zeviani, Giulia d'Amati

Research output: Contribution to journalArticle

Abstract

Mitochondrial (mt) diseases are multisystem disorders due to mutations in nuclear or mtDNA genes. Among the latter, more than 50% are located in transfer RNA (tRNA) genes and are responsible for a wide range of syndromes, for which no effective treatment is available at present. We show that three human mt aminoacyl-tRNA syntethases, namely leucyl-, valyl-, and isoleucyl-tRNA synthetase are able to improve both viability and bioenergetic proficiency of human transmitochondrial cybrid cells carrying pathogenic mutations in the mt-tRNAIle gene. Importantly, we further demonstrate that the carboxy-terminal domain of human mt leucyl-tRNA synthetase is both necessary and sufficient to improve the pathologic phenotype associated either with these "mild" mutations or with the "severe" m.3243A>G mutation in the mt-tRNALeu(UUR) gene. Furthermore, we provide evidence that this small, non-catalytic domain is able to directly and specifically interact in vitro with human mt-tRNALeu(UUR) with high affinity and stability and, with lower affinity, with mt-tRNAIle. Taken together, our results sustain the hypothesis that the carboxy-terminal domain of human mt leucyl-tRNA synthetase can be used to correct mt dysfunctions caused by mt-tRNA mutations.

Original languageEnglish
Pages (from-to)169-182
Number of pages14
JournalEMBO Molecular Medicine
Volume6
Issue number2
DOIs
Publication statusPublished - Feb 2014

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Keywords

  • Aminoacyl-tRNA synthetases
  • Mitochondrial disease
  • Mitochondrial tRNA mutations
  • Molecular therapy

ASJC Scopus subject areas

  • Molecular Medicine

Cite this

Perli, E., Giordano, C., Pisano, A., Montanari, A., Campese, A. F., Reyes, A., Ghezzi, D., Nasca, A., Tuppen, H. A., Orlandi, M., Di Micco, P., Poser, E., Taylor, R. W., Colotti, G., Francisci, S., Morea, V., Frontali, L., Zeviani, M., & d'Amati, G. (2014). The isolated carboxy-terminal domain of human mitochondrial leucyl-tRNA synthetase rescues the pathological phenotype of mitochondrial tRNA mutations in human cells. EMBO Molecular Medicine, 6(2), 169-182. https://doi.org/10.1002/emmm.201303198