The Juxtamembrane Regions of the Epidermal Growth Factor Receptor and gp185erbB-2 Determine the Specificity of Signal Transduction

Oreste Segatto, Fulvio Lonardo, Daniel Wexler, Francesca Fazioli, Jacalyn H. Pierce, Donald P. Bottaro, Morris F. White, Pier Paolo Di Fiore

Research output: Contribution to journalArticlepeer-review

Abstract

The epidermal growth factor receptor (EGFR) and gp185erbB-2 are closely related tyrosine kinases. Despite extensive sequence and structural homology, these two receptors display quantitative and qualitative differences in their ability to couple with mitogenic signalling pathways. By using chimeric molecules between EGFR and erbB-2, we found that the determinants responsible for the specificity of mitogenic signal transduction are located in the amino-terminal half of the tyrosine kinase domain of either receptor. In the EGFR, mutational analysis within this subdomain revealed that deletion of residues 660 to 667 impaired receptor mitogenic activity without affecting its tyrosine kinase properties. This sequence is therefore likely to contribute to the specificity of substrate recognition by the EGFR kinase.

Original languageEnglish
Pages (from-to)3191-3202
Number of pages12
JournalMolecular and Cellular Biology
Volume11
Issue number6
Publication statusPublished - Jun 1991

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

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