The latest advancements in proteomic two-dimensional gel electrophoresis analysis applied to biological samples

Laura Santucci, Maurizio Bruschi, Gian Marco Ghiggeri, Giovanni Candiano

Research output: Contribution to journalArticlepeer-review

Abstract

Two-dimensional gel electrophoresis (2DE) is one of the fundamental approaches in proteomics for the separation and visualization of complex protein mixtures. Proteins can be analyzed by 2DE using isoelectric focusing (IEF) in the first dimension, combined to sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) in the second dimension, gel staining (silver and Coomassie), image analysis, and 2DE gel database. High-resolution 2DE can resolve up to 5,000 different proteins simultaneously (∼2,000 proteins routinely), and detect and quantify

Original languageEnglish
Pages (from-to)103-125
Number of pages23
JournalMethods in molecular biology (Clifton, N.J.)
Volume1243
DOIs
Publication statusPublished - 2014

Keywords

  • Immobilized pH gradient
  • Isoelectric focusing
  • Protein staining
  • Proteomics
  • Sample preparation
  • Sodium dodecyl sulfate–polyacrylamide gel electrophoresis
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

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