The length of telomeric G-rich strand 3'-overhang measured by oligonucleotide ligation assay.

G. Cimino-Reale, E. Pascale, E. Battiloro, G. Starace, R. Verna, E. D'Ambrosio

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

A typical G-rich telomeric DNA strand, which runs 5'-->3' toward the chromosome ends, protrudes by several nucleotides in lower eukaryotes. In human chromosomes long G-rich 3'-overhangs have been found. Apart from the standard G-rich tail, several non-canonical terminal structures have been proposed. However, the mechanism of long-tail formation, the presence and the role of these structures in telomere maintenance or shortening are not completely understood. In a search for a simple method to accurately measure the 3'-overhang we have established a protocol based on the ligation of telomeric oligonucleotide hybridized to non-denatured DNA under stringent conditions (oligonucleotide ligation assay with telomeric repeat oligonucleotide). This method enabled us to detect a large proportion of G-rich single-stranded telomeric DNA that was as short as 24 nt. Nevertheless, we showed G-tails longer than 400 nt. In all tested cells the lengths ranging from 108 to 270 nt represented only 37% of the whole molecule population, while 56-62% were

Original languageEnglish
JournalNucleic Acids Research
Volume29
Issue number7
Publication statusPublished - Apr 1 2001

Fingerprint

Oligonucleotides
Ligation
Chromosomes, Human, 21-22 and Y
Chromosomes, Human, Pair 3
Single-Stranded DNA
DNA
Telomere
Human Chromosomes
Eukaryota
Nucleotides
Maintenance
Population

ASJC Scopus subject areas

  • Genetics

Cite this

Cimino-Reale, G., Pascale, E., Battiloro, E., Starace, G., Verna, R., & D'Ambrosio, E. (2001). The length of telomeric G-rich strand 3'-overhang measured by oligonucleotide ligation assay. Nucleic Acids Research, 29(7).

The length of telomeric G-rich strand 3'-overhang measured by oligonucleotide ligation assay. / Cimino-Reale, G.; Pascale, E.; Battiloro, E.; Starace, G.; Verna, R.; D'Ambrosio, E.

In: Nucleic Acids Research, Vol. 29, No. 7, 01.04.2001.

Research output: Contribution to journalArticle

Cimino-Reale, G, Pascale, E, Battiloro, E, Starace, G, Verna, R & D'Ambrosio, E 2001, 'The length of telomeric G-rich strand 3'-overhang measured by oligonucleotide ligation assay.', Nucleic Acids Research, vol. 29, no. 7.
Cimino-Reale G, Pascale E, Battiloro E, Starace G, Verna R, D'Ambrosio E. The length of telomeric G-rich strand 3'-overhang measured by oligonucleotide ligation assay. Nucleic Acids Research. 2001 Apr 1;29(7).
Cimino-Reale, G. ; Pascale, E. ; Battiloro, E. ; Starace, G. ; Verna, R. ; D'Ambrosio, E. / The length of telomeric G-rich strand 3'-overhang measured by oligonucleotide ligation assay. In: Nucleic Acids Research. 2001 ; Vol. 29, No. 7.
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