The leukocyte Ig-like receptor (LIR)-1 for the cytomegalovivus UL18 protein displays a broad specificity for different HLA class I alleles: Analysis of LIR-1+ NK cell clones

Massimo Vitale, Roberta Castriconi, Silvia Parolini, Daniela Pende, Mei Ling Hsu, Lorenzo Moretta, David Cosman, Alessandro Moretta

Research output: Contribution to journalArticle

Abstract

Leukocyte Ig-like receptor (LIR)-1 is a member of the Ig superfamily which has been shown to bind the human cytomegalovirus MHC class I homologue UL-18 protein. In this study, we have analyzed the expression and function of LIR-1 in human NK cells. We show that LIR-1 is expressed by a subset of NK cells variable in size among different donors. When compared to the known HLA class I-specific NK receptors, the expression of LIR-1 was found to be partially overlapped with that of CD94-NKG2A or with that of killer inhibitory receptors (KIR) belonging to the Ig superfamily. The use of the soluble form of UL-18 molecule revealed, in double fluorescence analysis, a selective binding to LIR-1+ cells while no correlation was observed between expression of either KIR or CD94-NKG2A molecules and ability to bind UL18. We further determined whether LIR-1 could also function as receptor for HLA class I molecules. To this end, we assessed the capability of LIR-1+ NK cell clones of lysing HLA class I- target cells transfected with different class I alleles, including HLA-A, -B, -C and -G alleles. Data revealed that LIR-1 functions as a broad HLA class I-specific inhibitory receptor recognizing different alleles coded for by different HLA loci.

Original languageEnglish
Pages (from-to)29-35
Number of pages7
JournalInternational Immunology
Volume11
Issue number1
DOIs
Publication statusPublished - 1999

Keywords

  • Cytotoxicity
  • HLA class I
  • Inhibitory receptors
  • NK cell

ASJC Scopus subject areas

  • Immunology

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