The LG11/Epitempin gene encodes two protein isoforms differentially expressed in human brain

Sandra Furlan, Federico Roncaroli, Francesca Forner, Libero Vitiello, Elisa Calabria, Salomé Piquer-Sirerol, Giorgio Valle, Jordi Perez-Tur, Roberto Michelucci, Carlo Nobile

Research output: Contribution to journalArticle

Abstract

The leucine-rich, glioma inactivated 1 (LGI1)/Epitempin gene has been linked to two phenotypes as different as gliomagenesis and autosomal dominant lateral temporal epilepsy. Its function and the biochemical features of the encoded protein are unknown. We characterized the LGI1/Epitempin protein product by western blot analysis of mouse and human brain tissues. Two proteins of about 60 and 65 kDa were detected by an anti-LGI1 antibody within the expected molecular mass range. The two proteins appeared to reside in different subcellular compartments, as they were fractionated by differential centrifugation. The specificity of both polypeptides was validated by cell transfection assay and mass spectrometry analysis. Immunoblot analysis of protein distribution in various zones of the human brain revealed variable amounts of both proteins. Notably, these proteins were more abundant in the temporal neocortex than in the hippocampus, the difference in abundance of the 65-kDa product being particularly pronounced. These results suggest that the two protein isoforms encoded by LGI1/Epitempin are differentially expressed in the human brain, and that higher expression levels of these proteins in the lateral temporal cortex may underlie the susceptibility of this brain region to the epileptogenic effects of LGI1/Epitempin mutations.

Original languageEnglish
Pages (from-to)985-991
Number of pages7
JournalJournal of Neurochemistry
Volume98
Issue number3
DOIs
Publication statusPublished - Aug 2006

Keywords

  • Brain localization
  • LGI1 protein
  • Temporal epilepsy
  • Western blot

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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    Furlan, S., Roncaroli, F., Forner, F., Vitiello, L., Calabria, E., Piquer-Sirerol, S., Valle, G., Perez-Tur, J., Michelucci, R., & Nobile, C. (2006). The LG11/Epitempin gene encodes two protein isoforms differentially expressed in human brain. Journal of Neurochemistry, 98(3), 985-991. https://doi.org/10.1111/j.1471-4159.2006.03939.x