The lipid A of Burkholderia multivorans C1576 smooth-type lipopolysaccharide and its pro-inflammatory activity in a cystic fibrosis airways model

Teresa Ieranò, Paola Cescutti, Maria Rosaria Leone, Alessandro Luciani, Roberto Rizzo, Valeria Raia, Rosa Lanzetta, Michelangelo Parrilli, Luigi Maiuri, Alba Silipo, Antonio Molinaro

Research output: Contribution to journalArticle

Abstract

Cystic fibrosis is an autosomal recessive disorder and it is characterised by chronic bacterial airway infection which leads to progressive lung deterioration, sometimes with fatal outcome. Burkholderia multivorans and Burkholderia cenocepacia are the species responsible for most of the infections of cystic fibrosis patients. Lipopolysaccharide endotoxins (LPSs) are among the foremost factors of pathogenesis of Gram-negative infection and, in particular, lipid A is the endotoxic portion of LPS responsible for eliciting host innate immune response. In this work, the complete primary structure of the lipid A from B. multivorans C1576 has been defined and, further, its pro-inflammatory activity in a cystic fibrosis airways model is shown. The structure of B. multivorans lipid A was attained by chemical, mass spectrometry and nuclear magnetic resonance analyses whereas its biological activity was assessed on the intestinal epithelial cell line CACO-2 cells, on the airway epithelial IB3-1 cells, carrying the ΔF508/W1282X CFTR mutation and on an ex vivo model of culture explants of nasal polyps.

Original languageEnglish
Pages (from-to)354-365
Number of pages12
JournalInnate Immunity
Volume16
Issue number6
DOIs
Publication statusPublished - Dec 2010

Keywords

  • Burkholderia multivorans
  • cystic fibrosis
  • lipid A
  • lipopolysaccharide
  • mass spectrometry

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Immunology
  • Microbiology
  • Infectious Diseases

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  • Cite this

    Ieranò, T., Cescutti, P., Leone, M. R., Luciani, A., Rizzo, R., Raia, V., Lanzetta, R., Parrilli, M., Maiuri, L., Silipo, A., & Molinaro, A. (2010). The lipid A of Burkholderia multivorans C1576 smooth-type lipopolysaccharide and its pro-inflammatory activity in a cystic fibrosis airways model. Innate Immunity, 16(6), 354-365. https://doi.org/10.1177/1753425909347400