The localization of human cyclins B1 and B2 determines CDK1 substrate specificity and neither enzyme requires MEK to disassemble the Golgi apparatus

Viji Mythily Draviam, Simona Orrechia, Martin Lowe, Ruggero Pardi, Jonathon Pines

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

In this paper, we show that substrate specificity is primarily conferred on human mitotic cyclin-dependent kinases (CDKs) by their subcellular localization. The difference in localization of the B-type cyclin-CDKs underlies the ability of cyclin B1-CDK1 to cause chromosome condensation, reorganization of the microtubules, and disassembly of the nuclear lamina and of the Golgi apparatus, while it restricts cyclin B2-CDK1 to disassembly of the Golgi apparatus. We identify the region of cyclin B2 responsible for its localization and show that this will direct cyclin B1 to the Golgi apparatus and confer upon it the more limited properties of cyclin B2. Equally, directing cyclin B2 to the cytoplasm with the NH2 terminus of cyclin B1 confers the broader properties of cyclin B1. Furthermore, we show that the disassembly of the Golgi apparatus initiated by either mitotic cyclin-CDK complex does not require mitogen-activated protein kinase kinase (MEK) activity.

Original languageEnglish
Pages (from-to)945-958
Number of pages14
JournalJournal of Cell Biology
Volume152
Issue number5
DOIs
Publication statusPublished - Mar 5 2001

Fingerprint

Cyclin B2
Cyclin B1
Mitogen-Activated Protein Kinase Kinases
Golgi Apparatus
Substrate Specificity
Cyclin-Dependent Kinases
Enzymes
Nuclear Lamina
Cyclin B
Cyclins
Microtubules
Cytoplasm
Chromosomes

Keywords

  • CDK
  • Cyclin
  • Golgi apparatus
  • Mitosis
  • Protein kinase

ASJC Scopus subject areas

  • Cell Biology

Cite this

The localization of human cyclins B1 and B2 determines CDK1 substrate specificity and neither enzyme requires MEK to disassemble the Golgi apparatus. / Draviam, Viji Mythily; Orrechia, Simona; Lowe, Martin; Pardi, Ruggero; Pines, Jonathon.

In: Journal of Cell Biology, Vol. 152, No. 5, 05.03.2001, p. 945-958.

Research output: Contribution to journalArticle

@article{7e1017326e3247cea77c32ab5a944c39,
title = "The localization of human cyclins B1 and B2 determines CDK1 substrate specificity and neither enzyme requires MEK to disassemble the Golgi apparatus",
abstract = "In this paper, we show that substrate specificity is primarily conferred on human mitotic cyclin-dependent kinases (CDKs) by their subcellular localization. The difference in localization of the B-type cyclin-CDKs underlies the ability of cyclin B1-CDK1 to cause chromosome condensation, reorganization of the microtubules, and disassembly of the nuclear lamina and of the Golgi apparatus, while it restricts cyclin B2-CDK1 to disassembly of the Golgi apparatus. We identify the region of cyclin B2 responsible for its localization and show that this will direct cyclin B1 to the Golgi apparatus and confer upon it the more limited properties of cyclin B2. Equally, directing cyclin B2 to the cytoplasm with the NH2 terminus of cyclin B1 confers the broader properties of cyclin B1. Furthermore, we show that the disassembly of the Golgi apparatus initiated by either mitotic cyclin-CDK complex does not require mitogen-activated protein kinase kinase (MEK) activity.",
keywords = "CDK, Cyclin, Golgi apparatus, Mitosis, Protein kinase",
author = "Draviam, {Viji Mythily} and Simona Orrechia and Martin Lowe and Ruggero Pardi and Jonathon Pines",
year = "2001",
month = "3",
day = "5",
doi = "10.1083/jcb.152.5.945",
language = "English",
volume = "152",
pages = "945--958",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "5",

}

TY - JOUR

T1 - The localization of human cyclins B1 and B2 determines CDK1 substrate specificity and neither enzyme requires MEK to disassemble the Golgi apparatus

AU - Draviam, Viji Mythily

AU - Orrechia, Simona

AU - Lowe, Martin

AU - Pardi, Ruggero

AU - Pines, Jonathon

PY - 2001/3/5

Y1 - 2001/3/5

N2 - In this paper, we show that substrate specificity is primarily conferred on human mitotic cyclin-dependent kinases (CDKs) by their subcellular localization. The difference in localization of the B-type cyclin-CDKs underlies the ability of cyclin B1-CDK1 to cause chromosome condensation, reorganization of the microtubules, and disassembly of the nuclear lamina and of the Golgi apparatus, while it restricts cyclin B2-CDK1 to disassembly of the Golgi apparatus. We identify the region of cyclin B2 responsible for its localization and show that this will direct cyclin B1 to the Golgi apparatus and confer upon it the more limited properties of cyclin B2. Equally, directing cyclin B2 to the cytoplasm with the NH2 terminus of cyclin B1 confers the broader properties of cyclin B1. Furthermore, we show that the disassembly of the Golgi apparatus initiated by either mitotic cyclin-CDK complex does not require mitogen-activated protein kinase kinase (MEK) activity.

AB - In this paper, we show that substrate specificity is primarily conferred on human mitotic cyclin-dependent kinases (CDKs) by their subcellular localization. The difference in localization of the B-type cyclin-CDKs underlies the ability of cyclin B1-CDK1 to cause chromosome condensation, reorganization of the microtubules, and disassembly of the nuclear lamina and of the Golgi apparatus, while it restricts cyclin B2-CDK1 to disassembly of the Golgi apparatus. We identify the region of cyclin B2 responsible for its localization and show that this will direct cyclin B1 to the Golgi apparatus and confer upon it the more limited properties of cyclin B2. Equally, directing cyclin B2 to the cytoplasm with the NH2 terminus of cyclin B1 confers the broader properties of cyclin B1. Furthermore, we show that the disassembly of the Golgi apparatus initiated by either mitotic cyclin-CDK complex does not require mitogen-activated protein kinase kinase (MEK) activity.

KW - CDK

KW - Cyclin

KW - Golgi apparatus

KW - Mitosis

KW - Protein kinase

UR - http://www.scopus.com/inward/record.url?scp=0035809921&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035809921&partnerID=8YFLogxK

U2 - 10.1083/jcb.152.5.945

DO - 10.1083/jcb.152.5.945

M3 - Article

C2 - 11238451

AN - SCOPUS:0035809921

VL - 152

SP - 945

EP - 958

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 5

ER -