The long-term prognostic value of survivin expressing circulating tumor cells in patients with high-risk non-muscle invasive bladder cancer (NMIBC)

Chiara Nicolazzo, Gian Maria Busetto, Francesco Del Giudice, Isabella Sperduti, Diana Giannarelli, Angela Gradilone, Paola Gazzaniga, Ettore de Berardinis, Cristina Raimondi

Research output: Contribution to journalArticlepeer-review

Abstract

OBJECTIVES: Long-term follow-up study to evaluate the impact on disease-free survival and cancer-specific survival of survivin expression in tissue and CTCs from T1G3 bladder cancer patients.

PATIENTS AND METHODS: The study was conducted using tumor tissue and blood samples from 54 patients with a primary diagnosis of T1G3 NMIBC. Survivin was evaluated by reverse transcription-polymerase chain reaction in tumor tissues. CTCs were isolated from blood by CELLection™ Dynabeads (Invitrogen, Carlsbad, CA, USA). Cells were lysed and cDNA was synthesized and analysed for the expression of CD45, CK8 and survivin. The endpoints of this long-termanalysis were disease-free survival, DFS and cancer-specific survival, CSS.

RESULTS: Here, we report that, at 9 years of median follow-up, disease-free survival and cancer-specific survival are both significantly influenced by the expression of survivin in tumor tissue (p = 0.006), by the presence of CTCs (p < 0.0001) and by the expression of survivin in CTCs (p < 0.0001).

CONCLUSION: The statistically significant impact of survivin expressing CTCs on cancer-specific survival that we observed might be interpreted as the result of the persistence of a subpopulation of highlander cells in the blood of T1G3 bladder patients over time.

Original languageEnglish
Pages (from-to)1971-1976
Number of pages6
JournalJournal of Cancer Research and Clinical Oncology
Volume143
Issue number10
DOIs
Publication statusPublished - Oct 2017

Keywords

  • Disease-Free Survival
  • Follow-Up Studies
  • Humans
  • Inhibitor of Apoptosis Proteins
  • Kaplan-Meier Estimate
  • Liquid Biopsy
  • Neoplastic Cells, Circulating
  • Prognosis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Urinary Bladder Neoplasms
  • Journal Article

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