The macrophage tetraspan MS4A4A enhances dectin-1-dependent NK cell–mediated resistance to metastasis

Irene Mattiola, Federica Tomay, Maria De Pizzol, Rita Silva-Gomes, Benedetta Savino, Tamara Gulic, Andrea Doni, Silvia Lonardi, Marie Astrid Boutet, Alessandra Nerviani, Roberta Carriero, Martina Molgora, Matteo Stravalaci, Diego Morone, Irina N. Shalova, Yunquin Lee, Subhra K. Biswas, Giovanna Mantovani, Marina Sironi, Costantino PitzalisWilliam Vermi, Barbara Bottazzi, Alberto Mantovani, Massimo Locati

Research output: Contribution to journalArticle

Abstract

The plasma membrane tetraspan molecule MS4A4A is selectively expressed by macrophage-lineage cells, but its function is unknown. Here we report that MS4A4A was restricted to murine and human mononuclear phagocytes and was induced during monocyte-to-macrophage differentiation in the presence of interleukin 4 or dexamethasone. Human MS4A4A was co-expressed with M2/M2-like molecules in subsets of normal tissue-resident macrophages, infiltrating macrophages from inflamed synovium and tumor-associated macrophages. MS4A4A interacted and colocalized with the β-glucan receptor dectin-1 in lipid rafts. In response to dectin-1 ligands, Ms4a4a-deficient macrophages showed defective signaling and defective production of effector molecules. In experimental models of tumor progression and metastasis, Ms4a4a deficiency in macrophages had no impact on primary tumor growth, but was essential for dectin-1-mediated activation of macrophages and natural killer (NK) cell–mediated metastasis control. Thus, MS4A4A is a tetraspan molecule selectively expressed in macrophages during differentiation and polarization, essential for dectin-1-dependent activation of NK cell–mediated resistance to metastasis.

Original languageEnglish
Pages (from-to)1012-1022
Number of pages11
JournalNature Immunology
Volume20
Issue number8
DOIs
Publication statusPublished - Aug 1 2019

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Macrophages
Neoplasm Metastasis
Neoplasms
Glucans
Macrophage Activation
dectin 1
Synovial Membrane
Phagocytes
Interleukin-4
Dexamethasone
Monocytes
Theoretical Models
Cell Membrane
Ligands
Lipids
Growth

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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The macrophage tetraspan MS4A4A enhances dectin-1-dependent NK cell–mediated resistance to metastasis. / Mattiola, Irene; Tomay, Federica; De Pizzol, Maria; Silva-Gomes, Rita; Savino, Benedetta; Gulic, Tamara; Doni, Andrea; Lonardi, Silvia; Astrid Boutet, Marie; Nerviani, Alessandra; Carriero, Roberta; Molgora, Martina; Stravalaci, Matteo; Morone, Diego; Shalova, Irina N.; Lee, Yunquin; Biswas, Subhra K.; Mantovani, Giovanna; Sironi, Marina; Pitzalis, Costantino; Vermi, William; Bottazzi, Barbara; Mantovani, Alberto; Locati, Massimo.

In: Nature Immunology, Vol. 20, No. 8, 01.08.2019, p. 1012-1022.

Research output: Contribution to journalArticle

Mattiola, I, Tomay, F, De Pizzol, M, Silva-Gomes, R, Savino, B, Gulic, T, Doni, A, Lonardi, S, Astrid Boutet, M, Nerviani, A, Carriero, R, Molgora, M, Stravalaci, M, Morone, D, Shalova, IN, Lee, Y, Biswas, SK, Mantovani, G, Sironi, M, Pitzalis, C, Vermi, W, Bottazzi, B, Mantovani, A & Locati, M 2019, 'The macrophage tetraspan MS4A4A enhances dectin-1-dependent NK cell–mediated resistance to metastasis', Nature Immunology, vol. 20, no. 8, pp. 1012-1022. https://doi.org/10.1038/s41590-019-0417-y
Mattiola, Irene ; Tomay, Federica ; De Pizzol, Maria ; Silva-Gomes, Rita ; Savino, Benedetta ; Gulic, Tamara ; Doni, Andrea ; Lonardi, Silvia ; Astrid Boutet, Marie ; Nerviani, Alessandra ; Carriero, Roberta ; Molgora, Martina ; Stravalaci, Matteo ; Morone, Diego ; Shalova, Irina N. ; Lee, Yunquin ; Biswas, Subhra K. ; Mantovani, Giovanna ; Sironi, Marina ; Pitzalis, Costantino ; Vermi, William ; Bottazzi, Barbara ; Mantovani, Alberto ; Locati, Massimo. / The macrophage tetraspan MS4A4A enhances dectin-1-dependent NK cell–mediated resistance to metastasis. In: Nature Immunology. 2019 ; Vol. 20, No. 8. pp. 1012-1022.
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