The major allergen of peach (Prunus persica) is a lipid transfer protein

Elide A. Pastorello, Laura Farioli, Valerio Pravettoni, Claudio Ortolani, Marco Ispano, Mara Monza, Chiara Baroglio, Elisabetta Scibola, Raffaella Ansaloni, Cristoforo Incorvaia, Amedeo Conti

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Allergy to fresh fruits and vegetables is mostly observed in subjects with pollinosis, especially from birch, because of cross-reacting allergens in vegetable foods and pollens. However, allergic reactions to fruits, specifically Rosaceae fruits, have been reported in subjects without pollinosis. Objective: This study evaluated the pattern of IgE reactivity, identifying the allergen responsible in 2 groups of patients with oral allergy syndrome to peach with or without birch pollinosis. Methods: The allergenic components of peach were detected by SDS-PAGE and immunoblotting. The major peach allergen was purified by HPLC with a cation-exchange column followed by gel filtration chromatography. Its IgE-binding capacity and its homology with the protein of the crude extract were demonstrated by immunoblotting inhibition techniques. To better characterize this allergen, periodic acid-Schiff stain and isoelectrofocusing were used. The amino acid sequencing was done with a gas-phase sequencer. Results: SDS-PAGE and immunoblotting of the 15 patients allergic to peach, 8 without and 7 with birch pollinosis, showed that they all recognized a protein with a molecular weight of 9 kd. This was the only allergen recognized by patients not sensitized to pollen, whereas the birch pollen-sensitive patients had IgE binding to other allergenic proteins at higher molecular weights. The purified 9-kd protein retained its IgE-binding capacity, was negative to periodic acid-Schiff stain, and had an isoelectric point value of greater than 9. A search in the Swiss Prot Bank showed this was a lipid transfer protein, belonging to a group of molecules involved in the defensive system of plants. Conclusions: The major allergen of peach is a 9-kd protein belonging to the group of lipid transfer proteins. This is the only allergen recognized by patients allergic to peach but not sensitized to birch pollen.

Original languageEnglish
Pages (from-to)520-526
Number of pages7
JournalJournal of Allergy and Clinical Immunology
Volume103
Issue number3 II
Publication statusPublished - 1999

Keywords

  • Birch pollinosis
  • Immunoblotting
  • Lipid transfer protein
  • Major allergen
  • Oral allergy syndrome
  • Peach

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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