The mammalian CHORD-containing protein melusin is a stress response protein interacting with Hsp90 and Sgt1

Mauro Sbroggiò, Roberta Ferretti, Elena Percivalle, Malgorzata Gutkowska, Alicja Zylicz, Wojciech Michowski, Jacek Kuznicki, Federica Accornero, Beniamina Pacchioni, Gerolamo Lanfranchi, Jorg Hamm, Emilia Turco, Lorenzo Silengo, Guido Tarone, Mara Brancaccio

Research output: Contribution to journalArticle

Abstract

Melusin is a mammalian muscle specific CHORD containing protein capable of activating signal transduction pathways leading to cardiomyocytes hypertrophy in response to mechanical stress. To define melusin function we searched for molecular partners possibly involved in melusin dependent signal transduction. Here we show that melusin and heat shock proteins are co-regulated. Moreover, melusin directly binds to Hsp90, a ubiquitous chaperone involved in regulating several signaling pathways. In addition, melusin interacts with Sgt1, an Hsp90 binding molecule. Melusin does not behave as an Hsp90 substrate but rather as a chaperone capable to protect citrate synthase from heat induced aggregation. These results describe melusin as a new component of the Hsp90 chaperone machinery. Structured summary: MINT-6538515:melusin (Q9R000)physically interacts (MI:0218) with Hsp90 (P07901) by anti bait co-immunoprecipitation (MI:0006)MINT-6538566, MINT-6538556:Hsp90 (P07901) physically interacts (MI:0218) with melusin (Q9R000) by cross-linking studies (MI:0030)MINT-6538524:melusin (Q9R000) physically interacts (MI:0218) with Sgt1 (Q9CS74) by anti tag co-immunoprecipitation (MI:0007)MINT-6538595:Hsp90 (P07901) physically interacts (MI:0218) with melusin (Q9R000) by enzyme linked immunosorbent assay (MI:0411)MINT-6538543:melusin (Q9R000) physically interacts (MI:0218) with Sgt1 (Q9CS74) by anti bait co-immunoprecipitation (MI:0006)MINT-6538580: melusin (Q9R000) physically interacts (MI:0218) with Hsp90 (P07901) by surface plasmon resonance (MI:0107).

Original languageEnglish
Pages (from-to)1788-1794
Number of pages7
JournalFEBS Letters
Volume582
Issue number13
DOIs
Publication statusPublished - Jun 11 2008

Keywords

  • CHORD containing proteins
  • Hsp90
  • Melusin
  • Sgt1

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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  • Cite this

    Sbroggiò, M., Ferretti, R., Percivalle, E., Gutkowska, M., Zylicz, A., Michowski, W., Kuznicki, J., Accornero, F., Pacchioni, B., Lanfranchi, G., Hamm, J., Turco, E., Silengo, L., Tarone, G., & Brancaccio, M. (2008). The mammalian CHORD-containing protein melusin is a stress response protein interacting with Hsp90 and Sgt1. FEBS Letters, 582(13), 1788-1794. https://doi.org/10.1016/j.febslet.2008.04.058