Abstract
The aim of the present study was to assess a suitable expression of the urinary concentration of a protein/peptide hormone such as insulin-like growth factor-I (IGF-I), measured in the urine of healthy individuals when the specimen collection is executed randomly. One hundred and twenty male subjects were divided by age into four groups, namely healthy sedentary young (SYA) and older (SOA) adults, older (OC) and young (YC) children. In a single urine specimen, randomly collected during the morning from each individual, total urinary IGF-I was measured by immunoradiometric method, and urinary creatinine (uCr) and total proteins (utPr) were measured by capillary electrophoresis and spectrophotometric methods, respectively. The urinary IGF-I concentrations were not significantly different in all groups investigated and they were (mean±SD): 82.7±82.8 ng/l, 103.5±83.3 ng/l, 80.4±64.4 ng/I in OC, SYA and SOA, respectively; only in the YC group there was a tendency to higher values (125.2.±93.2 ng/l) compared with the other groups. utPr ranged from 26 to 40 mg/l and did not demonstrate significant differences between groups. The urinary IGF-I correlated with uCr and utPr, and statistical significance was observed in all measurements. The measurement of urinary IGF-I in random urine and its ratio to utPr is an innovative, useful way of investigation of urinary protein/peptide hormones.
| Original language | English |
|---|---|
| Pages (from-to) | 574-578 |
| Number of pages | 5 |
| Journal | Clinical Chemistry and Laboratory Medicine |
| Volume | 40 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2002 |
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Keywords
- IGF-I
- Proteinuria
ASJC Scopus subject areas
- Clinical Biochemistry
Cite this
The measurement of insulin-like growth factor-I (IGF-I) concentration in random urine samples. / De Palo, Elio F.; Gatti, Rosalba; Lancerin, Federica; Cappellin, Enrico; Sartorio, Alessandro; Spinella, Paolo.
In: Clinical Chemistry and Laboratory Medicine, Vol. 40, No. 6, 2002, p. 574-578.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The measurement of insulin-like growth factor-I (IGF-I) concentration in random urine samples
AU - De Palo, Elio F.
AU - Gatti, Rosalba
AU - Lancerin, Federica
AU - Cappellin, Enrico
AU - Sartorio, Alessandro
AU - Spinella, Paolo
PY - 2002
Y1 - 2002
N2 - The aim of the present study was to assess a suitable expression of the urinary concentration of a protein/peptide hormone such as insulin-like growth factor-I (IGF-I), measured in the urine of healthy individuals when the specimen collection is executed randomly. One hundred and twenty male subjects were divided by age into four groups, namely healthy sedentary young (SYA) and older (SOA) adults, older (OC) and young (YC) children. In a single urine specimen, randomly collected during the morning from each individual, total urinary IGF-I was measured by immunoradiometric method, and urinary creatinine (uCr) and total proteins (utPr) were measured by capillary electrophoresis and spectrophotometric methods, respectively. The urinary IGF-I concentrations were not significantly different in all groups investigated and they were (mean±SD): 82.7±82.8 ng/l, 103.5±83.3 ng/l, 80.4±64.4 ng/I in OC, SYA and SOA, respectively; only in the YC group there was a tendency to higher values (125.2.±93.2 ng/l) compared with the other groups. utPr ranged from 26 to 40 mg/l and did not demonstrate significant differences between groups. The urinary IGF-I correlated with uCr and utPr, and statistical significance was observed in all measurements. The measurement of urinary IGF-I in random urine and its ratio to utPr is an innovative, useful way of investigation of urinary protein/peptide hormones.
AB - The aim of the present study was to assess a suitable expression of the urinary concentration of a protein/peptide hormone such as insulin-like growth factor-I (IGF-I), measured in the urine of healthy individuals when the specimen collection is executed randomly. One hundred and twenty male subjects were divided by age into four groups, namely healthy sedentary young (SYA) and older (SOA) adults, older (OC) and young (YC) children. In a single urine specimen, randomly collected during the morning from each individual, total urinary IGF-I was measured by immunoradiometric method, and urinary creatinine (uCr) and total proteins (utPr) were measured by capillary electrophoresis and spectrophotometric methods, respectively. The urinary IGF-I concentrations were not significantly different in all groups investigated and they were (mean±SD): 82.7±82.8 ng/l, 103.5±83.3 ng/l, 80.4±64.4 ng/I in OC, SYA and SOA, respectively; only in the YC group there was a tendency to higher values (125.2.±93.2 ng/l) compared with the other groups. utPr ranged from 26 to 40 mg/l and did not demonstrate significant differences between groups. The urinary IGF-I correlated with uCr and utPr, and statistical significance was observed in all measurements. The measurement of urinary IGF-I in random urine and its ratio to utPr is an innovative, useful way of investigation of urinary protein/peptide hormones.
KW - IGF-I
KW - Proteinuria
UR - http://www.scopus.com/inward/record.url?scp=0036339238&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036339238&partnerID=8YFLogxK
U2 - 10.1515/CCLM.2002.099
DO - 10.1515/CCLM.2002.099
M3 - Article
VL - 40
SP - 574
EP - 578
JO - Clinical Chemistry and Laboratory Medicine
JF - Clinical Chemistry and Laboratory Medicine
SN - 1434-6621
IS - 6
ER -