The mechanism of docosahexaenoic acid-induced phospholipase D activation in human lymphocytes involves exclusion of the enzyme from lipid rafts

Olivier Diaz, Alexandre Berquand, Madeleine Dubois, Di Silvia Agostino, Claudio Sette, Sylvain Bourgoin, Michel Lagarde, Georges Némoz, Annie France Prigent

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Docosahexaenoic acid (DHA), an n-3 polyunsaturated fatty acid that inhibits T lymphocyte activation, has been shown to stimulate phospholipase D (PLD) activity in stimulated human peripheral blood mononuclear cells (PBMC). To elucidate the mechanisms underlying the DHA-induced PLD activation, we first characterized the PLD expression pattern of PBMC. We show that these cells express PLD1 and PLD2 at the protein and mRNA level and are devoid of oleate-dependent PLD activity. DHA enrichment of PBMC increased the DHA content of cell phospholipids, which was directly correlated with the extent of PLD activation. The DHA-induced PLD activation was independent of conventional protein kinase C but inhibited by brefeldin A, which suggests ADP-ribosylation factor (ARF)-dependent mechanism. Furthermore, DHA enrichment dose-dependently stimulated ARF translocation to cell membranes. Whereas 50% of the guanosine 5′-3-O-(thio)triphosphate plus ARF-dependent PLD activity and a substantial part of PLD1 protein were located to the detergent-insoluble membranes, so-called rafts, of non-enriched PBMC, DHA treatment strongly displaced them toward detergent-soluble membranes where ARF is present. Collectively, these results suggest that the exclusion of PLD1 from lipid rafts, due to their partial disorganization by DRA,DHA, and its relocalization in the vicinity of ARF, is responsible for its activation. This PLD activation might be responsible for the immunosuppressive effect of DHA because it is known to transmit antiproliferative signals in lymphoid cells.

Original languageEnglish
Pages (from-to)39368-39378
Number of pages11
JournalJournal of Biological Chemistry
Volume277
Issue number42
DOIs
Publication statusPublished - Oct 18 2002

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Phospholipase D
Lymphocytes
Docosahexaenoic Acids
ADP-Ribosylation Factors
Chemical activation
Lipids
Enzymes
Blood Cells
Blood
Detergents
Guanosine 5'-O-(3-Thiotriphosphate)
Membranes
Brefeldin A
T-cells
Omega-3 Fatty Acids
Cell membranes
Oleic Acid
Immunosuppressive Agents
Lymphocyte Activation
Protein Kinase C

ASJC Scopus subject areas

  • Biochemistry

Cite this

The mechanism of docosahexaenoic acid-induced phospholipase D activation in human lymphocytes involves exclusion of the enzyme from lipid rafts. / Diaz, Olivier; Berquand, Alexandre; Dubois, Madeleine; Agostino, Di Silvia; Sette, Claudio; Bourgoin, Sylvain; Lagarde, Michel; Némoz, Georges; Prigent, Annie France.

In: Journal of Biological Chemistry, Vol. 277, No. 42, 18.10.2002, p. 39368-39378.

Research output: Contribution to journalArticle

Diaz, Olivier ; Berquand, Alexandre ; Dubois, Madeleine ; Agostino, Di Silvia ; Sette, Claudio ; Bourgoin, Sylvain ; Lagarde, Michel ; Némoz, Georges ; Prigent, Annie France. / The mechanism of docosahexaenoic acid-induced phospholipase D activation in human lymphocytes involves exclusion of the enzyme from lipid rafts. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 42. pp. 39368-39378.
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T1 - The mechanism of docosahexaenoic acid-induced phospholipase D activation in human lymphocytes involves exclusion of the enzyme from lipid rafts

AU - Diaz, Olivier

AU - Berquand, Alexandre

AU - Dubois, Madeleine

AU - Agostino, Di Silvia

AU - Sette, Claudio

AU - Bourgoin, Sylvain

AU - Lagarde, Michel

AU - Némoz, Georges

AU - Prigent, Annie France

PY - 2002/10/18

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N2 - Docosahexaenoic acid (DHA), an n-3 polyunsaturated fatty acid that inhibits T lymphocyte activation, has been shown to stimulate phospholipase D (PLD) activity in stimulated human peripheral blood mononuclear cells (PBMC). To elucidate the mechanisms underlying the DHA-induced PLD activation, we first characterized the PLD expression pattern of PBMC. We show that these cells express PLD1 and PLD2 at the protein and mRNA level and are devoid of oleate-dependent PLD activity. DHA enrichment of PBMC increased the DHA content of cell phospholipids, which was directly correlated with the extent of PLD activation. The DHA-induced PLD activation was independent of conventional protein kinase C but inhibited by brefeldin A, which suggests ADP-ribosylation factor (ARF)-dependent mechanism. Furthermore, DHA enrichment dose-dependently stimulated ARF translocation to cell membranes. Whereas 50% of the guanosine 5′-3-O-(thio)triphosphate plus ARF-dependent PLD activity and a substantial part of PLD1 protein were located to the detergent-insoluble membranes, so-called rafts, of non-enriched PBMC, DHA treatment strongly displaced them toward detergent-soluble membranes where ARF is present. Collectively, these results suggest that the exclusion of PLD1 from lipid rafts, due to their partial disorganization by DRA,DHA, and its relocalization in the vicinity of ARF, is responsible for its activation. This PLD activation might be responsible for the immunosuppressive effect of DHA because it is known to transmit antiproliferative signals in lymphoid cells.

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