The methyl-CpG binding transcriptional repressor MeCP2 stably associates with nucleosomal DNA

Simon P. Chandler, Dmitry Guschin, Nicoletta Landsberger, Alan P. Wolffe

Research output: Contribution to journalArticle

Abstract

We have investigated the interactions of the methyl-CpG binding transcriptional repressor MeCP2 with nucleosomal DNA. We find that MeCP2 forms discrete complexes with nucleosomal DNA associating with methyl-CpGs exposed in the major groove via the methyl-CpG-binding domain (MBD). In addition to the MBD, the carboxyl-terminal segment of MeCP2 facilitates binding both to naked DNA and to the nucleosome core. These observations provide a molecular mechanism by which MeCP2 can gain access to chromatin in order to target corepressor complexes that further modify chromatin structure.

Original languageEnglish
Pages (from-to)7008-7018
Number of pages11
JournalBiochemistry
Volume38
Issue number22
DOIs
Publication statusPublished - Jun 1 1999

ASJC Scopus subject areas

  • Biochemistry

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    Chandler, S. P., Guschin, D., Landsberger, N., & Wolffe, A. P. (1999). The methyl-CpG binding transcriptional repressor MeCP2 stably associates with nucleosomal DNA. Biochemistry, 38(22), 7008-7018. https://doi.org/10.1021/bi990224y