The microRNA let-7b-5p Is Negatively Associated with Inflammation and Disease Severity in Multiple Sclerosis

Georgia Mandolesi, Francesca Romana Rizzo, Sara Balletta, Mario Stampanoni Bassi, Luana Gilio, Livia Guadalupi, Monica Nencini, Alessandro Moscatelli, Colleen Patricia Ryan, Valerio Licursi, Ettore Dolcetti, Alessandra Musella, Antonietta Gentile, Diego Fresegna, Silvia Bullitta, Silvia Caioli, Valentina Vanni, Krizia Sanna, Antonio Bruno, Fabio ButtariChiara Castelli, Carlo Presutti, Francesca De Santa, Annamaria Finardi, Roberto Furlan, Diego Centonze, Francesca De Vito

Research output: Contribution to journalArticlepeer-review

Abstract

The identification of microRNAs in biological fluids for diagnosis and prognosis is receiving great attention in the field of multiple sclerosis (MS) research but it is still in its infancy. In the present study, we observed in a large sample of MS patients that let-7b-5p levels in the cerebrospinal fluid (CSF) were highly correlated with a number of microRNAs implicated in MS, as well as with a variety of inflammation-related protein factors, showing specific expression patterns coherent with let-7b-5p-mediated regulation. Additionally, we found that the CSF let-7b-5p levels were significantly reduced in patients with the progressive MS compared to patients with relapsing-remitting MS and were negatively correlated with characteristic hallmark processes of the two phases of the disease. Indeed, in the non-progressive phase, let-7b-5p inversely associated with both central and peripheral inflammation; whereas, in progressive MS, the CSF levels of let-7b-5p negatively correlated with clinical disability at disease onset and after a follow-up period. Overall, our results uncovered, by the means of a multidisciplinary approach and multiple statistical analyses, a new possible pleiotropic action of let-7b-5p in MS, with potential utility as a biomarker of MS course.

Original languageEnglish
JournalCells
Volume10
Issue number2
DOIs
Publication statusPublished - Feb 5 2021

Keywords

  • Expanded Disability Status Scale (EDSS)
  • G_CSF
  • IL5
  • inflammation
  • let-7
  • miRNAs
  • multiple sclerosis (MS)
  • progressive multiple sclerosis (PMS)
  • RANTES

ASJC Scopus subject areas

  • Medicine(all)

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