The mitochondrial trigger in an animal model of nonalcoholic fatty liver disease

Guglielmina Chimienti, Antonella Orlando, Francesco Russo, Benedetta D’attoma, Manuela Aragno, Eleonora Aimaretti, Angela Maria Serena Lezza, Vito Pesce

Research output: Contribution to journalArticlepeer-review

Abstract

Nonalcoholic fatty liver disease (NAFLD) is the leading liver chronic disease featuring hepatic steatosis. Mitochondrial β-oxidation participates in the derangement of lipid metabolism at the basis of NAFLD, and mitochondrial oxidative stress contributes to the onset of the disease. We evaluated the presence and effects of mitochondrial oxidative stress in the liver from rats fed a high-fat plus fructose (HF-F) diet inducing NAFLD. Supplementation with dehydroepiandrosterone (DHEA), a multitarget antioxidant, was tested for efficacy in delaying NAFLD. A marked mitochondrial oxidative stress was originated by all diets, as demonstrated by the decrease in Superoxide Dismutase 2 (SOD2) and Peroxiredoxin III (PrxIII) amounts. All diets induced a decrease in mitochondrial DNA content and an increase in its oxidative damage. The diets negatively affected mitochondrial biogenesis as shown by decreased peroxisome proliferator-activated receptor-γ co-activator-1α (PGC-1α), mitochondrial transcription factor A (TFAM), and the COX-IV subunit from the cytochrome c oxidase complex. The reduced amounts of Beclin-1 and lipidated LC3 II form of the microtubule-associated protein 1 light chain 3 (LC3) unveiled the diet-related autophagy’s decrease. The DHEA supplementation did not prevent the diet-induced changes. These results demonstrate the relevance of mitochondrial oxidative stress and the sequential dysfunction of the organelles in an obesogenic diet animal model of NAFLD.

Original languageEnglish
Article number1439
JournalGenes
Volume12
Issue number9
DOIs
Publication statusPublished - Sep 2021

Keywords

  • DHEA
  • High fat-fructose diet
  • Mitochondrial biogenesis
  • MtDNA damage
  • NAFLD

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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