The mode of action of Y13-259 scFv fragment intracellularly expressed in mammalian cells

Alessio Cardinale, Matteo Lener, Samantha Messina, Antonino Cattaneo, Silvia Biocca

Research output: Contribution to journalArticle

Abstract

The anti-p21ras Y13-259 single-chain Fv fragment (scFv) neutralizes the activity of p21-ras when intracellularly expressed in different systems. We have studied the mode of action of this inhibition in 3T3 K-ras fibroblasts and demonstrated that (i) this antibody fragment is highly aggregating when cytoplasmically expressed and (ii) the p21-ras antigen is sequestered in these aggregates in an antibody-dependent manner. This co-segregation leads to an efficient inhibition of DNA synthesis. These results suggest that an antigen can be diverted from its normal location inside the cells in an antibody mediated way, prospecting a new mode of action for intracellular antibodies in vivo. Copyright (C) 1998 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)197-202
Number of pages6
JournalFEBS Letters
Volume439
Issue number3
DOIs
Publication statusPublished - Nov 20 1998

Keywords

  • Anti-p21ras antibody
  • Intracellular antibody
  • Protein solubility
  • scFv fragment

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint Dive into the research topics of 'The mode of action of Y13-259 scFv fragment intracellularly expressed in mammalian cells'. Together they form a unique fingerprint.

  • Cite this