The modulation of intracellular glutathione level modulates the mitochondrial response in proliferating rat splenocytes

Carlo Pieri, Maurizio Marra, Fausto Moroni, Fiorella Marcheselli, Cristiana Benatti

Research output: Contribution to journalArticle

Abstract

We have investigated the effect of intracellular reduced glutathione (GSH) levels on the mitochondrial parameters of proliferating rat splenocytes. The intracellular GSH content of the cells was altered by continuous culture with buthionine-S, R-sulfoximine (BSO), a specific inhibitor of GSH synthesis. BSO decreased the GSH levels, inhibited DNA synthesis and caused depolarization of mitochondria in 52% of cells stimulated to proliferate for 72 h. These data support the proposition that GSH influences the lymphocyte proliferation at more than one site, one of which is the energy producing machinery.

Original languageEnglish
Pages (from-to)115-125
Number of pages11
JournalArchives of Gerontology and Geriatrics
Volume21
Issue number2
DOIs
Publication statusPublished - 1995

Keywords

  • Glutathione
  • Membrane potential
  • Mitochondria
  • Splenocytes

ASJC Scopus subject areas

  • Ageing
  • Geriatrics and Gerontology
  • Gerontology
  • Health(social science)
  • Medicine(all)

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