The monosialoganglioside GM1 induces internalisation and degradation of the CD4 antigen in U937 cells: Evidence for a novel mechanism of CD4 down- modulation in a p56(lck)-negative cell line, which is independent of protein kinase C activation

C. Sorio, D. Saggioro, L. Chieco-Bianchi, G. Berton

Research output: Contribution to journalArticle

Abstract

Sialated glycosphingolipids (gangliosides) were recently shown to induce internalisation of the CD4 Ag in lymphoid cells and dissociation of p56(lck) from CD4 (Repke et al. (1992) J. Immunol. 149, 2585-2591; Saggioro et al. (1993) J. Biol. Chem. 268, 1368-1375). The findings presented in this paper show that GM1 induces internalisation and the eventual degradation of the CD4 Ag also in the monocytic cell line U937. GM1 effects are independent of a possible activation of protein kinase C, as enzyme inhibitors which effectively blocked phorbol esters effects did not prevent GM1-induced CD4 internalisation and degradation. GM1 effects were also independent of a possible action on a CD4 associated kinase activity as we show that U937 cells lack any CD4-associated kinase activity.

Original languageEnglish
Pages (from-to)1105-1110
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume191
Issue number3
DOIs
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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