The mouse aldehyde oxidase gene: Molecular cloning, chromosomal mapping and functional characterization of the 5'-flanking region

Silvia Demontis, Mami Kurosaki, Salvatore Saccone, Salvatore Motta, Enrico Garattini, Mineko Terao

Research output: Contribution to journalArticlepeer-review

Abstract

In this article, we report on the chromosome mapping and molecular cloning of the genetic locus encoding the mouse molybdo-iron/sulfur-flavoprotein aldehyde oxidase. The aldehyde oxidase locus maps to mouse chromosome 1 band C1-C2, as determined by fluorescence in situ hybridization experiments conducted on metaphase chromosomes. The gene is approximately 83 kb long and consists of 35 exons. The exon/intron boundaries are perfectly conserved relative to the corresponding human homolog and almost completely conserved relative to the mouse xanthine oxidoreductase gene. This further supports the concept that the aldehyde oxidase and xanthine oxidoreductase loci evolved from the same ancestral precursor by a gene duplication event. The position of a major transcription start site was defined by primer extension and RNase mapping analysis. The 5'-flanking region of the mouse aldehyde oxidase gene contains a functional and orientation-dependent promoter as well as several putative binding sites for known cell-specific and general transcription factors. Deletion analysis of the 5'-flanking region defines an approximately 470 bp DNA stretch which is necessary and sufficient for the transcription of the mouse aldehyde oxidase gene. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)207-222
Number of pages16
JournalBBA - Gene Structure and Expression
Volume1489
Issue number2-3
DOIs
Publication statusPublished - Dec 23 1999

Keywords

  • Aldehyde oxidase
  • Chromosome mapping
  • Gene
  • Molecular cloning
  • Mouse

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology

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