The murine Fhit locus: Isolation, characterization, and expression in normal and tumor cells

Yuri Pekarsky, Teresa Druck, Maria Grazia Cotticelli, Masataka Ohta, Jiang Shou, Jeannine Mendrola, Jeffry C. Montgomery, Arthur M. Buchberg, Linda D. Siracusa, Giacomo Manenti, Louise Y Y Fong, Tommaso A. Dragani, Carlo M. Croce, Kay Huebner

Research output: Contribution to journalArticle

Abstract

The murine Fhit locus maps near the centromere v proximal Ptprg locus on mouse chromosome 14. The cDNA sequence and structure are similar to those of the human gene, with exons 5-9 encoding the protein. The predominant mRNA in the tissues and cell lines tested was an alternatively spliced form missing exon 3. Most murine cell lines tested, including lines established from normal mouse embryos and tumors, expressed very low or undetectable levels of Fhit mRNA. Most normal mouse tissues expressed wild-type Fhit mRNA, whereas ~40% of murine lung carcinomas expressed wild-type and aberrant Fhit RT-PCR products that lacked various exons. Several tumorigenic mouse cell lines exhibited homozygous deletions of Fhit exons. We conclude that the murine Fhit gene, like its human counterpart, is a target of alterations involved in murine carcinogenesis.

Original languageEnglish
Pages (from-to)3401-3408
Number of pages8
JournalCancer Research
Volume58
Issue number15
Publication statusPublished - Aug 1 1998

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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